Summary of Study ST001272

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000856. The data can be accessed directly via it's Project DOI: 10.21228/M81T3M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001272
Study TitleGrowth cone memebrane and growth cone particulate lipidomics
Study Typeuntargeted LC-MS/MS lipidomics
Study SummaryWe performed high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the proteome and lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP)
Institute
University of Miami
DepartmentOphthalmology, Bascom Palmer Eye Institute
LaboratorySanjoy K. Bhattacharya
Last NameBhattacharya
First NameSanjoy
Address1638 NW 10th Avenue, Miami, FL 33136
EmailSBhattacharya@med.miami.edu
Phone305-482-4103
Submit Date2019-10-21
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-01-03
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M81T3M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001336
Collection Summary:Growth cone from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP) were collected. These fractions were generated through established differential centrifugation techniques and lipid extraction from established protocols: 1-Estrada-Bernal, A. et al. Functional complexity of the axonal growth cone: a proteomic analysis. PloS one 7, e31858 (2012). 2-Pfenninger et al 1983 paper (Pfenninger, K. H., Ellis, L., Johnson, M. P., Friedman, L. B. & Somlo, S. Nerve growth cones isolated from fetal rat brain: subcellular fractionation and characterization. Cell 35, 573-584 (1983)
Sample Type:Brain
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