Summary of Study ST001305

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000886. The data can be accessed directly via it's Project DOI: 10.21228/M85D77 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001305
Study TitleIntegrated Metabolomics and Transcriptomics Suggest the Global Metabolic Response to 2-Aminoacrylate Stress in Salmonella enterica
Study SummaryNMR metabolomics of bacterial extractions from WT and 2-iminobutanoate/2iminopropanoate deaminase (RidA) KO S. Enterica lines
Institute
University of Georgia
DepartmentCCRC
Last NameEdison
First NameArthur
Address315 Riverbend Road, Athens, GA 30602
Emailaedison@uga.edu
Phone7065428156
Submit Date2020-01-09
Num Groups2
Total Subjects19
Raw Data AvailableYes
Analysis Type DetailNMR
Release Date2020-03-03
Release Version1
Arthur Edison Arthur Edison
https://dx.doi.org/10.21228/M85D77
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Collection:

Collection ID:CO001374
Collection Summary:Ten biologically independent cultures each of wild-type (DM9404) and ridA mutant (DM3480) strains were grown overnight in NB medium at 37 °C and used to inoculate (1% inoculum) 250 mL minimal glucose medium in 500 mL non-baffled flasks. Flasks were randomly arranged in an Innova®44 incubator and cultures were allowed to grow 16 h shaking at 180 RPM and 37 °C. Cultures were cooled on ice 5 min and then harvested by centrifugation at 7,000 x G for 10 min at 4 °C. The supernatant was decanted, pellets were resuspended in 10 mL ddH2O and transferred to sterile 15 mL conical tubes in which they were pelleted at 7,000 x G 10 min at 4 °C. Final supernatant was decanted and pellets were frozen in liquid nitrogen and stored at -80 °C prior to cell extractions.
Sample Type:Bacterial cells
Storage Conditions:-80℃
  logo