Summary of study ST001380

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000944. The data can be accessed directly via it's Project DOI: 10.21228/M8P41V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001380
Study TitleFast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample specific ion distributions
Study SummaryMass spectrometry based metabolomics is a widely used approach in biotechnology and biomedical research. However, current methods coupling mass spectrometry with chromatography are time-consuming and not suitable for high-throughput analysis of thousands of samples. An alternative approach is flow-injection mass spectrometry (FI-MS) in which samples are directly injected to the ionization source. Here, we show that the sensitivity of Orbitrap FI-MS metabolomics methods is limited by ion competition effect in the detection system. We describe an approach for overcoming this effect by analyzing the distribution of ion m/z values and computationally determining a series of optimal scan ranges. This enables reproducible detection of ~9,000 and ~10,000 m/z features in metabolomics and lipidomics analysis of serum samples, respectively, with a sample scan time of ~15 seconds and duty time of ~30 seconds; a ~50% increase versus current spectral-stitching FI-MS. This approach facilitates high-throughput metabolomics for a variety of applications, including biomarker discovery and functional genomics screens.
Institute
Technion – Israel Institute of Technology
Last NameShlomi
First NameTomer
AddressTechnion
Emailtomersh@cs.technion.ac.il
Phone+972-77-8871497
Submit Date2020-05-17
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2020-05-22
Release Version1
Tomer Shlomi Tomer Shlomi
https://dx.doi.org/10.21228/M8P41V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001449
Collection Summary:FI-MS method optimization was performed with commercially available serum, Human AB Serum (Biological Industries USA, Inc., USA). Matrix effect experiments and biological applications were performed with 98 serum samples of healthy individuals obtained from Rambam Hospital, Haifa, Israel (IRB 0481-18-RMB).
Sample Type:Blood (serum)
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