Summary of Study ST001394
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000957. The data can be accessed directly via it's Project DOI: 10.21228/M80D7P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001394 |
| Study Title | Metabolomic study of Escherichia coli K-12 MG1655 and mutants |
| Study Type | Metabolomics |
| Study Summary | Metabolomic analysis of Wildtype, crp mutant and its five adaptively evolved populations evolved in glucose minimal media with 40 mM MOPS during its exponential phase of growth. Three biological and two technical replicate samples (n=6) were harvested for each of the strains while growing in a bioreactor aerobically at 37 degree Celsius and 700 rpm. This study aims to characterize and compare the metabolic profile of all these strains. |
| Institute | IIT Bombay |
| Department | Department of Chemical Engineering |
| Laboratory | Systems Biology and Metabolic Engineering Laboratory |
| Last Name | Pal |
| First Name | Ankita |
| Address | IIT Bombay, Powai, Mumbai - 400076, Maharashtra, India |
| pal.ankita90@gmail.com | |
| Phone | 08879536816 |
| Submit Date | 2020-06-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-03-01 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001463 |
| Collection Summary: | The Wildtype, crp mutant and the five evolved replicate populations were grown in a bioreactor aerobically in glucose minimal media with 40 mM MOPS at 37 degrees Celsius and 700 rpm. The samples were harvested during its exponential phase of growth for three biological and two technical replicates (n=6). The metabolites were extracted using the Methanol-Chloroform-Water method. Detailed sample collection protocol is described in “extraction_method.pdf”. |
| Sample Type: | Bacterial cells |
| Collection Method: | Methanol-Chloroform-Water method |
| Collection Location: | IIT Bombay, Powai, Mumbai- 400076, Mumbai, Maharashtra |
| Storage Conditions: | -80℃ |
