Summary of Study ST001421

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000975. The data can be accessed directly via it's Project DOI: 10.21228/M8NX1G This work is supported by NIH grant, U2C- DK119886.

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Study IDST001421
Study TitleMetabolomic study of Escherichia coli K-12 MG1655 WT and its transcriptional regulator mutants under anaerobic fermentation conditions
Study TypeMetabolomics
Study SummaryMetabolomic analysis of Wildtype, fnr, arcA and ihf mutants in glucose minimal media under anaerobic fermentation conditions during its exponential phase of growth. Three biological and two technical replicate samples (n=6) were harvested for each of the strains while growing in a bioreactor anaerobically at 37 degrees Celsius and 150 rpm. This study aims to characterize and compare the metabolic profiles of all these strains.
Institute
IIT Bombay
DepartmentDepartment of Chemical Engineering
LaboratorySystems Biology and Metabolic Engineering Laboratory (SBMEL)
Last NamePal
First NameAnkita
AddressDepartment of Chemical Engineering, IIT Bombay, Mumbai, Maharashtra, 400076, India
Emailpal.ankita90@gmail.com
Phone08879536816
Submit Date2020-07-07
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-03-01
Release Version1
Ankita Pal Ankita Pal
https://dx.doi.org/10.21228/M8NX1G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001490
Collection Summary:The WT, fnr, arcA and ihf mutants were grown in a bioreactor in glucose minimal media under anaerobic fermentation conditions at 37 degrees Celsius and 150 rpm. The samples were harvested during its exponential phase of growth for three biological and two technical replicates (n=6). The metabolites were extracted using the Methanol-Chloroform-Water method. Detailed sample collection protocol is described in “Extraction_protocol_msi.pdf”.
Collection Protocol Filename:ankita_pal_Extraction_protocol_msi.pdf
Sample Type:Bacterial cells
Collection Method:Methanol-Chloroform-Water method
Collection Location:IIT Bombay, Powai, Mumbai- 400076, Mumbai, Maharashtra
Storage Conditions:-80℃
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