Summary of Study ST001527
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001028. The data can be accessed directly via it's Project DOI: 10.21228/M8T99V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001527 |
| Study Title | Lung cancer metabolomics analysis |
| Study Summary | This study explored models predictive of staging and chemotherapy response based on metabolomic analysis of fresh, patient-derived non-small cell lung cancer (NSCLC) core biopsies. Prospectively collected tissue samples before initial treatment were evaluated with high-resolution 2DLC-MS/MS and 13C-glucose enrichment, and the data were comprehensively analyzed with machine learning techniques. Patients were categorized as Disease-Control (DC) [encompassing complete-response (CR), partial-response (PR), and stable-disease (SD)] and Progressive-Disease (PD). Four major types of learning methods (partial least squares discriminant analysis (PLS-DA), support vector machines (SVM), artificial neural networks, and random forests) were applied to differentiate between positive (DC and CR/PR) and poor (PD and SD/PD) responses, and between stage I/II/III and stage IV disease. Models were trained with forward feature selection based on variable importance and tested on validation subsets. |
| Institute | University of Louisville |
| Department | Bioengineering |
| Last Name | Frieboes |
| First Name | Hermann |
| Address | Lutz Hall 419 |
| hbfrie01@louisville.edu | |
| Phone | 502-852-3302 |
| Submit Date | 2020-09-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-08-01 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001596 |
| Collection Summary: | Tumor tissue samples were placed in a 1.8mL cryovial with either DMEM or RPMI cell culture medium and kept on ice during transport. In the laboratory, they were immediately placed into 1mL 13C labeled glucose medium in 24-well cell culture plate and incubated at 5% CO2 and 37oC on a shaker for 24h. |
| Sample Type: | Tumor cells |
