Summary of Study ST001609

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001034. The data can be accessed directly via it's Project DOI: 10.21228/M81X28 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001609
Study TitleComparative metabolomics analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p (part-I)
Study SummaryTo gather more in-depth knowledge of the Mtl1p mechanosensor's role in Saccharomyces cerevisiae metabolism, we conducted a comparative metabolomic analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p. Both strains were grown under normal conditions at 27°C. The most significant metabolic changes between these strains were related to amino acid metabolism, purine metabolism, and carboxylic acid metabolism.
Institute
University of Puerto Rico, Medical Sciences Campus
Last NameChorna
First NameNataliya
AddressUniversity of Puerto Rico, Medical Sciences Campus, Department of Biochemistry, Main Building, 6th Floor, Room A-632, San Juan, PR 00935
Emailnataliya.chorna@upr.edu
Phone787-758-2525 ext 1640
Submit Date2020-11-27
Num Groups2
Total Subjects26
Raw Data AvailableYes
Raw Data File Type(s)qgd
Analysis Type DetailGC-MS
Release Date2020-12-10
Release Version1
Nataliya Chorna Nataliya Chorna
https://dx.doi.org/10.21228/M81X28
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001679
Collection Summary:Cells were grown at 27°C, 210 rpm in 5 mL of complete synthetic medium (CSM, 0.67% Nitrogen base without amino acids and ammonium sulfate, 2% glucose). The next day, cells were replaced with fresh CSM and grow at 27°C to reach an OD of 0.6 - 0.7. 25 ml of each sample were harvested by centrifugation at 3,838 x g for 5 min at 4°C, washed with 1 ml ice cold sterile water, quenched using ice cold methanol, and stored at -80°C.
Sample Type:Yeast cells
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