Summary of Study ST001623
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001041. The data can be accessed directly via it's Project DOI: 10.21228/M84M6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001623 |
| Study Title | Targeted metabolomics analysis with raffinose-rich diet in mouse ileum |
| Study Type | Analysis of fructose level in ileum of stressed mouse fed with raffinose-rich diet |
| Study Summary | Purified diet (AIN-93G) supplemented with raffinose was prepared. The mice were maintained on the separate diet for at least 1 week before the initiation of experiment.Chronic restraint stress (RS) in mice was performed 14 days.After sacrifice, the ileum of mice were used to analysis. |
| Institute | China Pharmaceutical University |
| Department | School of Medicine |
| Laboratory | Metabonomics |
| Last Name | Hou |
| First Name | Yuanlong |
| Address | tongjiaxiang, nanjing, jiangsu, 210000, China |
| jian2103@163.com | |
| Phone | 18851105337 |
| Submit Date | 2020-12-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-12-16 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001693 |
| Collection Summary: | jejunum tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 ℃) and 100 µL of H2O (pre-cooled at 4℃) was added for homogenate for 1 min. 1 μg/mL 4-chloro-phenylalanine and 800 µL methanol solution were added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 30000g for 10 min at 4 °C, and dried under vacuum. It was reconstituted with 100 μL of methanol: water solution (50:50, v/v) before analysis. |
| Sample Type: | Intestine |
