Summary of Study ST001633

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001031. The data can be accessed directly via it's Project DOI: 10.21228/M8F39C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001633
Study TitleAromatic amino acid metabolism by the anaerobic gut bacterium Clostridium sporogenes (part-II)
Study SummaryGerm-free MCAD-/- mice or MCAD+/+ controls were mono-colonized with wild-type Clostridium sporogenes. Urine was collected for untargeted LC-QTOF analysis.
Institute
Stanford University
LaboratoryDodd
Last NamePruss
First NameKali
Address300 Pasteur Drive, Lane 235
Emailkmpruss@stanford.edu
Phone6507212961
Submit Date2020-12-11
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2021-01-26
Release Version1
Kali Pruss Kali Pruss
https://dx.doi.org/10.21228/M8F39C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001703
Collection Summary:MCAD-/- mice were re-derived as germ-free (GF) for the purpose of this study. MCAD-/- mice or MCAD+/+ controls were maintained in sterile gnotobiotic isolators or cages with HEPA-filtered air supply. Urine from GF MCAD-/- and MCAD+/+ mice was collected under sterile conditions. Seven days subsequent, mice were mono-colonized with wild-type C. sporogenes (Cs) by gavage of 200 µL saturated overnight culture. Urine was collected one week after colonization. C. sporogenes colonization was verified with serial dilution plating. Urine samples were immediately frozen on dry ice and stored under -80 oC until analysis.
Sample Type:Urine
Storage Conditions:-80℃
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