Summary of Study ST001850

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001167. The data can be accessed directly via it's Project DOI: 10.21228/M8VM4C This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001850
Study TitleUnbiased LC-MS-based metabolomics analysis for both whole cell and mitochondria metabolites to gain an insight into the role of Tug1/PGC1 axis on metabolite profiles in podocytes
Study Summaryunbiased LC-MS-based metabolomics analysis for both whole cell and mitochondria metabolites to gain an insight into the role of Tug1/PGC1 axis on metabolite profiles in podocytes
Institute
University of Texas MD Anderson Cancer Center
Last NameDanesh
First NameFarhad
Address1515 Holcombe Blvd, Houston ,TX77030
Emailfdanesh@mdanderson.org
Phone7135634498
Submit Date2021-06-25
Num Groups3
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-06-29
Release Version1
Farhad Danesh Farhad Danesh
https://dx.doi.org/10.21228/M8VM4C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001920
Collection Summary:Briefly, podocytes were cultured on BD BioCoat Collagen I plates (BD Biosciences, San Jose, CA) at 33°C in RPMI 1640 complete media with 20 U/ml mouse recombinant IFN-g (Thermo Fisher, Carlsbad, CA). To induce differentiation, we cultured podocytes in DMEM (5.5mM glucose and 5% FBS) at 37°C without IFN-g for 10-12 days. To rescue the expression of PGC1-a in stable Tug1-knockdown CRISPR clone (Tug1-KD)(Long et al., 2016), CMV enhancer/promoter-driven mouse Pgc1-a cDNA (Addgene, Watertown, MA) was inserted into vector Zeo-pT-MCS-GFP-T2A-Puro(Long et al., 2020), a modified PiggyBac transposon system, selected with 1ug/ml puromycin and sorted by GFP, to generate Tug1-KD/Pgc1-OE. We isolated kidney podocytes by positive selection with biotin-labelled Kirrel3 and Podocalyxin antibodies (2.5 μg/antibody/mouse, R&D Systems, Minneapolis, MN) followed by Streptavidin M-280 Dynabeads as previously described (Badal et al., 2016).
Sample Type:Epithelial cells
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