Summary of Study ST001865

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001178. The data can be accessed directly via it's Project DOI: 10.21228/M8F99F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST001865
Study TitleSystemic metabolite changes due to Hypoxia
Study TypeComparative metabolomic analysis of serum metabolites detected by untargeted LC/MS and GC/MS platform
Study SummaryProlonged cellular hypoxia leads to energetic failure and death. However, sublethal hypoxia can trigger an adaptive response called hypoxic preconditioning. While prolyl-hydroxylase (PHD) enzymes and hypoxia inducible factors (HIFs) have been identified as key elements of oxygen sensing machinery, the mechanisms by which hypoxic preconditioning protects against insults remain unclear. Here, we perform serum metabolomic profiling to assess alterations induced by hypoxic preconditioning. We discover that hypoxic preconditioning increases serum kynurenine levels and enhance kynurenine biotransformation leading to preservation of NAD+ in the post-ischemic kidney. Furthermore, we show that Indoleamine 2,3-dioxygenase 1 (Ido1) deficiency abolishes the systemic increase of kynurenine and the subsequent renoprotection generated by hypoxic preconditioning. Importantly, exogenous administration of kynurenine restores the hypoxic preconditioning in the context of Ido1 deficiency. Collectively, our findings demonstrate a critical role of Ido1/kynurenine axis in mediating hypoxic preconditioning
Institute
Northwestern University
DepartmentMedicine/Nephrology
LaboratoryKapitsinou
Last NameKapitsinou
First NamePinelopi
Address303 East Superior Street
Emailpinelopi.kapitsinou@northwestern.edu
Phone312-503-8710
Submit Date2021-07-01
Num Groups2
Total Subjects16
Num Males16
Study CommentsN/A
PublicationsAccepted in Cell Reports
Chear StudyNo
Analysis Type DetailLC-MS
Release Date2022-01-02
Release Version1
Pinelopi Kapitsinou Pinelopi Kapitsinou
https://dx.doi.org/10.21228/M8F99F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Collection:

Collection ID:CO001935
Collection Summary:Whole blood was collected in BD Microtainer Serum Separator Tubes. After collection of the whole blood, blood was allowed to clot by leaving it undisturbed at room temperature for 20 minutes. Clot was removed by centrifuging at 1,000–2,000 x g for 10 minutes in a refrigerated centrifuge. The resulting supernatant(serum) was transferred into clean polypropylene tubes and stored at –80°C until time of analysis.
Sample Type:Blood (serum)
  logo