Summary of Study ST001903
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001198. The data can be accessed directly via it's Project DOI: http://dx.doi.org/10.21228/M8VD7F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001903 |
| Study Title | Effect of ketogenic diet on the plasma and tumor metabolome of melanoma-bearing mice |
| Study Type | Metabolomics analysis |
| Study Summary | Growing evidence supports the use of low-carbohydrate/high-fat ketogenic diets (KDs) together with standard therapies to improve cancer treatment outcomes. However, conflicting data exist regarding the efficacy of KDs in melanoma, the deadliest skin cancer. Here, we show that two different KD formulations effectively reduced tumor growth in immunocompromised mice bearing genetically and metabolically heterogeneous human melanoma xenografts. Furthermore, the KDs exerted a metastasis-reducing effect in an immunocompetent syngeneic melanoma mouse model. Ketone bodies did not directly influence melanoma cell proliferation; therefore, we performed an in-depth metabolomics analysis using the MxP® Quant 500 kit combined with a acylcarnitine assay (biocrates life sciences ag)to elucidate potential anti-tumor mechanisms in vivo. Targeted analysis of plasma and tumor metabolomes revealed distinct changes in amino acid metabolism induced by the KDs. Moreover, the KDs increased sphingomyelin synthesis and hydroxylation of certain lipids. Thus, KDs simultaneously affect multiple metabolic pathways to create an unfavorable environment for melanoma cell proliferation, supporting their potential as a complementary nutritional approach to melanoma therapy. |
| Institute | University Hospital of the Paracelsus Medical University Salzburg |
| Last Name | Weber |
| First Name | Daniela |
| Address | Müllner Hauptstraße 48, 5020 Salzburg, Austria |
| d.weber@salk.at | |
| Phone | 0043 57255 26274 |
| Submit Date | 2021-08-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS/MS(Dir. Inf.) |
| Release Date | 2022-07-11 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002417 |
| Collection Summary: | Human melanoma xenografts were established in mice using A375 and WM47 (BRAF mutant), WM3311 (BRAF/NRAS wild-type) and WM3000 (NRAS mutant) cells. Blood was taken by cardiac puncture, collected in heparin-coated tubes and centrifuged for 3 min at 2000 g. Plasma samples were stored at -80 °C until analysis. Tumors were harvested and snap frozen in liquid nitrogen and stored at -80°C for metabolomics. |
| Sample Type: | Blood (plasma) and tumor tissue |
