Summary of Study ST002104

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001333. The data can be accessed directly via it's Project DOI: 10.21228/M8DM7G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002104
Study TitleChemoresistant Cancer Cell Lines are Characterized by Migratory, Amino Acid Metabolism, Protein Catabolism and IFN1 Signalling Perturbations
Study TypeBiomedical research
Study SummaryOur analysis was able to separate chemoresistant cells from their parental cells based on their metabolomic and proteomic features and identified altered biological processes and pathways which are of further interest. Preliminary investigation of patient-derived cells highlighted the need to perform broad biological and molecular analyses, compre-hensive in vitro and in vivo studies, using a larger patient cohort to achieve a deeper and clinically relevant characterization of the molecular drivers of chemoresistance.
Institute
Future Industries Institute
LaboratoryManuela Klingler-Hoffmann
Last NameAcland
First NameMitchell
AddressX Building, Mawson Lakes South Australia 5095
Emailmitch.acland@gmail.com
Phone0448671141
Submit Date2022-02-06
Num Groups4
Total Subjects12
Num MalesNA
Num FemalesNA
Study CommentsOVCAR5 and CaOV3 cell lines and their carboplatin resistant cell lines
PublicationsChemoresistant Cancer Cell Lines are Characterized by Migra-tory, Amino Acid Metabolism, Protein Catabolism and IFN1 Signalling Perturbations
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-03-29
Release Version1
Mitchell Acland Mitchell Acland
https://dx.doi.org/10.21228/M8DM7G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002182
Collection Summary:Cells were maintained at 60-80% confluence for 3 passages before being plated in 10cm dishes. Cell numbers were estimated from an additional dish with the same number of cells at seeding. Media was aspirated and cells were washed three times with 3mL warmed PBS. Metabolic arrest was achieved through the addition of approximately 2 mL of liquid nitrogen directly to cells ensuring that the surface of the plate was covered before plates were placed onto dry ice.
Sample Type:Cultured cells
Storage Conditions:-80℃
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