Summary of Study ST002578

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001663. The data can be accessed directly via it's Project DOI: 10.21228/M8R72W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002578
Study TitleSteady State Metabolomics Study in SMARCA4 mutant(BIN-67) cells cultured in the absence of glucose or glutamine
Study Summarysteady-state abundances of all TCA cycle intermediates were more severely reduced in BIN-67 cells deprived of glutamine than those deprived of glucose
Institute
McGill University
DepartmentBiochemistry
LaboratorySidong Huang Lab
Last NameFu
First NameZheng
AddressMcIntyre Medical Sciences Building, 3655 promenade Sir-William-Osler
Emailzheng.fu2@mail.mcgill.ca
Phone5143985446
Submit Date2023-04-24
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailGC-MS
Release Date2023-05-22
Release Version1
Zheng Fu Zheng Fu
https://dx.doi.org/10.21228/M8R72W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002673
Collection Summary:BIN-67 cells were plated in RPMI supplemented with 6% dialyzed FBS (Wisent Bio, Cat# 080-950) at ~80% confluency the day before experiments. The next day, BIN-67 cells were cultured in the absence of glucose or glutamine for 24h. Cells were washed twice in cold saline solution (NaCl, 0.9 g/l) and metabolites were extracted with 1 ml 80% ice-cold methanol (GC/MS grade).
Sample Type:Ovarian cancer cells
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