Summary of Study ST002956
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001839. The data can be accessed directly via it's Project DOI: 10.21228/M80T6F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002956 |
| Study Title | Automated preparation of plasma lipids, metabolites, and proteins for LC/MS-based analysis of a high-fat diet in mice |
| Study Type | Method Development |
| Study Summary | We designed an automated liquid-liquid extraction method with minimal contamination and human intervention. This approach enables accurate and precise collection of metabolite, lipid fractions, and protein pellet from a small volume of mice plasma for multiomic analysis. |
| Institute | Calico Life Sciences |
| Department | Department of Mass Spectrometry-Technology Lab |
| Laboratory | Metabolomics Lab |
| Last Name | Vu |
| First Name | Ngoc |
| Address | 1130 Veterans BLVD |
| ngoc@calicolabs.com | |
| Phone | 6504205430 |
| Submit Date | 2023-07-06 |
| Num Groups | 3 |
| Total Subjects | 90 |
| Num Males | 90 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-29 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003062 |
| Collection Summary: | Each mouse was fasted four hours prior to each of three retro orbital bleeds performed over three weeks at 10 AM (Time point 1- Z10), 10 PM (Time point 2- Z22), and 3 PM (Time point 3- Z15). Blood was collected into BD microtainer tubes with K2E (K2EDTA), mixed briefly, and stored on ice. All samples were centrifuged at 7000 RCF for 4 mins at 4°C to separate blood plasma. Plasma was aliquoted in 50 µL increments, immediately frozen on dry ice, and stored at -80C for later analysis. |
| Collection Protocol ID: | A00003888 |
| Collection Protocol Filename: | NIH Guide for the Care and Use of Laboratory Animals |
| Sample Type: | Blood (plasma) |
| Collection Method: | retro bleed |
| Collection Location: | Calico Life Sciences |
| Collection Frequency: | every week |
| Storage Conditions: | -80℃ |
| Collection Vials: | BD microcontainer tube with K2E |
