Summary of Study ST003333
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002072. The data can be accessed directly via it's Project DOI: 10.21228/M8SF9X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003333 |
| Study Title | Urine Metabolite Profiling in Indian Male Population at High Altitude using Liquid Chromatography – Mass Spectrometry: A Longitudinal Pilot Study |
| Study Summary | People who visit high altitude (HA) for work, pilgrimage, recreation purposes or deployment are subjected to environmental stresses such as low temperature, atmospheric pressure leading to hypoxia, high radiations, dry air, non-availability of fresh food and vegetables, etc. These environmental stressors pose significant physiological effects on the human body. Among these challenges, hypobaric hypoxia at high altitude affects the aerobic metabolism and thereby reducing the supply of metabolic energy. These alterations could further lead to extreme environment-related maladaptation as evidenced by changes in metabolites and metabolic pathways. In order to investigate the variation in metabolite prfile, urine samples were collected from 16 individual at base line (BL) and at High altitude (HA, 4420m). Untargeted urine metabolic profiling was performed using liquid chromatography–mass spectrometry (LC-MS) in conjunction with statistical analysis. Univariate and Multivariate statistical analysis revealed 33 metabolites based on fold change, (FC >2 and < 0.5), VIP score (>1) and p value (<0.05). These 33 metabolites were primarily associated with pathways related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Biotin metabolism, Cysteine and methionine metabolism, Tyrosine metabolism, Alanine, aspartate and glutamate metabolism, Pentose and glucuronate interconversions, Vitamin B6 metabolism, Citrate cycle (TCA cycle) and Porphyrin metabolism. Further, ROC analysis detected 5 metabolites i.e., 2-Tetrahydrothiopheneacetic acid (AUC: 0.97266), 1-Benzyl-7,8-dimethoxy-3-phenyl-3H-pyrazolo[3,4-c]isoquinoline (AUC: 0.92969), Abietin (AUC:0.917), 4,4'-Thiobis-2-butanone (AUC: 0.917), and Hydroxyisovaleroyl carnitine (AUC: 0.910) with a high range of sensitivity and specificity. In summary, this present longitudinal study demonstrated metabolic changes in human exposed to high altitude, via utilising the potential application of LC-MS metabolomics. These findings will shed light on the impact of hypoxic exposure on metabolic adaptation and provide a better understanding about the pathophysiological mechanism of HA related illnesses correlated to tissue hypoxia. |
| Institute | Defence Institute of Physiology and Allied Sciences |
| Last Name | Meena |
| First Name | Ramesh Chand |
| Address | Lucknow Road, Timarpur |
| rcmeena.dipas@gov.in | |
| Phone | 9868784269 |
| Submit Date | 2024-07-12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-08-08 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003447 |
| Collection Summary: | First urine pass of the morning (120 ml), was collected in sterile urine vials. The initial sample collection took place at base line, denoted as BL (210 m). After the BL collection, participants travelled to different altitude (9000m-15000m) for acclimatization and reached at high altitude, 4420m (HA), and final collection was done after their stay for on month. Within 1 h of collection, all the samples were immediately treated with Sodium Azide (2.5mM), centrifuged for 10 min at 4000 rpm for removal of particles. The samples were immediately distributed in aliquots of 2 ml each and stored at −40 °C. The sample were then transported to BL, stored at −80 °C for further acquisition and analysis. |
| Sample Type: | Urine |
| Collection Tube Temp: | -80 degree |
