Summary of Study ST003371

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002085. The data can be accessed directly via it's Project DOI: 10.21228/M83R6P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003371
Study TitleIncorporation of oleate-d9 and elaidate-d17 into membrane lipids of Huh7 cells.
Study SummaryWe analyzed several membrane lipid classes including LPC, LPE, PC, and PE in Huh7 cells treated with BSA-oleate-d9 or BSA-elaidate-d17 to determine their incorporation into the broader lipidome.
Institute
Salk Institute for Biological Studies
Last NameGengatharan
First NameJivani
Address10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA
Emailjivani14@gmail.com
Phone(858) 453-4100
Submit Date2024-07-30
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2024-08-12
Release Version1
Jivani Gengatharan Jivani Gengatharan
https://dx.doi.org/10.21228/M83R6P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO003485
Collection Summary:Cells (~400,000 cells)were spiked with 1 µg of each of the following internal standards: 15:0-18:1(d7) phosphatidylcholine (Avanti Polar Lipids, Cat #791637), 15:0-18:1(d7) phosphatidylethanolamine (Avanti Polar Lipids, Cat #791638), 18:1(d7) lysophosphatidylcholine (Avanti Polar Lipids, Cat#791643), 18:1(d7) lysophosphatidylethanolamine (Avanti Polar Lipids, Cat #791644), 15:0-18:1(d7) diacylglycerol (Avanti Polar Lipids, Cat #791647), 15:0-18:1(d7)-15:0 triacylglycerol (Avanti Polar Lipids, Cat #791648). Cells were scraped with 0.5 mL methanol and 0.5 mL H2O.
Sample Type:Cultured cells
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