Summary of Study ST003484
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002140. The data can be accessed directly via it's Project DOI: 10.21228/M80R74 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003484 |
| Study Title | Metabolism of TNBC cell line MDA-MB-453 is altered by cytokines, TDO2 inhibition, or suspension growth conditions - cellular steady state metabolome and in vitro tracing with 13C11 tryptophan |
| Study Summary | TNBC cell line MDA-MB-453 was cultured in 13C11 tryptophan and experimental treatments were as follows: a) cytokine treatment (TNFα+IL1β); b) treated with TDO2/IDO1 dual inhibitor AT-0174 for 24 or 48 hours with an without labeled tryptophan; c) cultured in 13C11 tryptophan and suspension condition. |
| Institute | University of Colorado Anschutz Medical Campus |
| Last Name | Haines |
| First Name | Julie |
| Address | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
| julie.haines@cuanschutz.edu | |
| Phone | 3037243339 |
| Submit Date | 2024-09-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-10-03 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003605 |
| Collection Summary: | The cells were detached using 0.5% trypsin/EDTA and then centrifuged at 1500rpm for 5 mins at 4℃, and the supernatants were discarded. Next, PBS was used to resuspend the cell pellets and centrifuged again at 13000rpm for 15 mins at 4℃. The supernatant then discarded. The cell pellets were frozen at -80℃. |
| Sample Type: | Breast cancer cells |
