Summary of Study ST003492

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002141. The data can be accessed directly via it's Project DOI: 10.21228/M8VZ4T This work is supported by NIH grant, U2C- DK119886.

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Study IDST003492
Study TitleMetabolomic profiling of the secretome in normal primary human pulmonary artery smooth muscle cells
Study SummaryGrowth medium of human pulmonary artery smooth muscle cells (PASMCs) was conditioned for 24 hours and analyzed by LC-MS, termed conditioned medium (CM). Comparing to fresh complete vascular smooth muscle cell growth medium (GM), LC-MS profiling screened 138 metabolites and identified that three branched chain alpha-ketoacids, namely KIC, KIV, and KMV are the most abundant metabolites in CM of human PASMCs.
Institute
Peking University
DepartmentDepartment of Toxicology
Last NameXiao
First NameWusheng
Address38 Xueyuan Rd, Haidian District, Beijing, Beijing, 100191, China
Emailwxiao@bjmu.edu.cn
Phone+1-010-82801628
Submit Date2023-08-07
Analysis Type DetailMALDI
Release Date2024-10-04
Release Version1
Wusheng Xiao Wusheng Xiao
https://dx.doi.org/10.21228/M8VZ4T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO003613
Collection Summary:Human primary pulmonary artery smooth muscle cells were cultured in complete vascular smooth muscle cell growth medium in a T75 flask. When cells reach to roungly 80% confluence, growth medium were refreshed with 10 mL and cultured for 24 hours. Medium samples were then collected and used for LC-MS analysis after centrifugation and removal of cell debris.
Sample Type:Vascular smooth muscle cells
Storage Conditions:-80℃
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