Summary of Study ST003808
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002382. The data can be accessed directly via it's Project DOI: 10.21228/M8QZ7Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003808 |
| Study Title | Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division |
| Study Summary | Peroxisomes play a crucial role in cellular metabolism. Glucose-6-phosphate dehydrogenase (G6PD), the gatekeeper enzyme of the pentose phosphate pathway, is primarily localized in the cytosol. However, studies have reported its presence in peroxisomes as well. This project aims to determine the function of G6PD on the peroxisomal membrane. In this study, we overexpressed G6PD in either the cytosol or on the peroxisomal membrane of mammary epithelial stem-like cells (hMECs). By comparing their lipidomic profiles, we found that peroxisomal membrane-associated G6PD provides NADPH, which feeds into peroxisomal ether lipid synthesis. |
| Institute | University of Helsinki |
| Department | Faculty of Biological and Environmental Sciences |
| Laboratory | Katajisto Laboratory |
| Last Name | Hien |
| First Name | Bui |
| Address | Biocenter 1, Viikinkaari 9 |
| hien.bui@helsinki.fi | |
| Phone | +358294159407 |
| Submit Date | 2025-03-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, mzdata.xml |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2025-03-24 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003935 |
| Collection Summary: | Human mammary epithelial cells (hMECs) line FL2 (PMID: 21498687) was used for this study. hMECs were maintained in MEGM mammary epithelial medium (Lonza, CC-3153). G6PD overexpression plasmids were generated in a pRP backbone by inserting the G6PD sequence with or without peroxisomal targeting signal downstream of the hPGK promoter. In addition, the plasmids contain an mCherry sequence downstream of an IRES sequence. mCherry was used as the selection marker for transfected cells in FACS sorting. To over-express G6PD in hMECs, endotoxin-free plasmids were transfected to the cells using jetPRIME (Polyplus, 101000015). 24hours post-transfection, cells were trypsinized, sorted into culture media by Fluorescence-Activated Cell Sorting (FACS), centrifuged at 500g for 5min, washed 1 with cold PBS following by washed 1 with sucrose 0.25M. Pellets were snap freeze with liquid N2 and store at -70. |
| Sample Type: | Epithelial cells |
