Summary of Study ST000092

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000084. The data can be accessed directly via it's Project DOI: 10.21228/M8PK5V This work is supported by NIH grant, U2C- DK119886.

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Study IDST000092
Study TitleA statistical analysis of the effects of urease pre-treatment on the measurement of the urinary metabolome by gas chromatography–mass spectrometry
Study TypeAnalytical Comparison
Study SummaryUrease pre-treatment of urine has been utilized since the early 1960s to remove high levels of urea from samples prior to further processing and analysis by gas chromatography–mass spectrometry (GC–MS). Aside from the obvious depletion or elimination of urea, the effect, if any, of urease pre-treatment on the urinary metabolome has not been studied in detail. Here, we report the results of three separate but related experiments that were designed to assess possible indirect effects of urease pre-treatment on the urinary metabolome as measured by GC–MS. In total, 235 GC–MS analyses were performed and over 106 identified and 200 unidentified metabolites were quantified across the three experiments. The results showed that data from urease pre-treated samples (1) had the same or lower coefficients of variance among reproducibly detected metabolites, (2) more accurately reflected quantitative differences and the expected ratios among different urine volumes, and (3) increased the number of metabolite identifications. Overall, we observed no negative consequences of urease pre-treatment. In contrast, urease pre-treatment enhanced the ability to distinguish between volume-based and biological sample types compared to no treatment. Taken together, these results show that urease pre-treatment of urine offers multiple beneficial effects that outweigh any artifacts that may be introduced to the data in urinary metabolomics analyses.
Institute
Pacific Northwest National Laboratory
DepartmentBiological Separation and Mass Spectrometry
Last NameMetz
First NameThomas
Emailthomas.metz@pnnl.gov
Submit Date2014-06-25
Num Groups6
Total Subjects235
Raw Data AvailableYes
Raw Data File Type(s)cdf
Uploaded File Size3.0 G
Analysis Type DetailGC-MS
Release Date2014-08-07
Release Version1
Thomas Metz Thomas Metz
https://dx.doi.org/10.21228/M8PK5V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Urease Treatment Volume Urine (µL) Gender Solution Added Ethnicity
SA005020VaryingVolume_NT_100uL_1No treatment 100 N/A N/A N/A
SA005021VaryingVolume_NT_100uL_3No treatment 100 N/A N/A N/A
SA005022VaryingVolume_NT_100uL_2No treatment 100 N/A N/A N/A
SA005023ConstantVolume_NT_4_3No treatment 100 N/A None N/A
SA005024ConstantVolume_NT_4_2No treatment 100 N/A None N/A
SA005025ConstantVolume_NT_5_1No treatment 100 N/A None N/A
SA005026ConstantVolume_NT_5_3No treatment 100 N/A None N/A
SA005027ConstantVolume_NT_1_1No treatment 100 N/A None N/A
SA005028ConstantVolume_NT_4_1No treatment 100 N/A None N/A
SA005029ConstantVolume_NT_5_2No treatment 100 N/A None N/A
SA005030ConstantVolume_NT_2_1No treatment 100 N/A None N/A
SA005031ConstantVolume_NT_1_3No treatment 100 N/A None N/A
SA005032ConstantVolume_NT_3_3No treatment 100 N/A None N/A
SA005033ConstantVolume_NT_1_2No treatment 100 N/A None N/A
SA005034ConstantVolume_NT_2_2No treatment 100 N/A None N/A
SA005035ConstantVolume_NT_3_2No treatment 100 N/A None N/A
SA005036ConstantVolume_NT_3_1No treatment 100 N/A None N/A
SA005037ConstantVolume_NT_2_3No treatment 100 N/A None N/A
SA005038VaryingVolume_NT_10uL_3No treatment 10 N/A N/A N/A
SA005039VaryingVolume_NT_10uL_1No treatment 10 N/A N/A N/A
SA005040VaryingVolume_NT_10uL_2No treatment 10 N/A N/A N/A
SA005041VaryingVolume_NT_25uL_2No treatment 25 N/A N/A N/A
SA005042VaryingVolume_NT_25uL_3No treatment 25 N/A N/A N/A
SA005043VaryingVolume_NT_25uL_1No treatment 25 N/A N/A N/A
SA005044MaleVsFemale_NT_Female_Asian_24_BRH600219_DNo treatment 50 Female N/A Asian
SA005045MaleVsFemale_NT_Female_Asian_23_BRH600227_BNo treatment 50 Female N/A Asian
SA005046MaleVsFemale_NT_Female_Asian_24_BRH600219_CNo treatment 50 Female N/A Asian
SA005047MaleVsFemale_NT_Female_Asian_23_BRH600227_ANo treatment 50 Female N/A Asian
SA005048MaleVsFemale_NT_Female_Black_24_BRH600216_BNo treatment 50 Female N/A Black
SA005049MaleVsFemale_NT_Female_Black_24_BRH600226_CNo treatment 50 Female N/A Black
SA005050MaleVsFemale_NT_Female_Black_24_BRH600216_ANo treatment 50 Female N/A Black
SA005051MaleVsFemale_NT_Female_Black_24_BRH600226_DNo treatment 50 Female N/A Black
SA005052MaleVsFemale_NT_Female_Caucasian_24_BRH600211_ANo treatment 50 Female N/A Caucasian
SA005053MaleVsFemale_NT_Female_Caucasian_24_BRH600211_BNo treatment 50 Female N/A Caucasian
SA005054MaleVsFemale_NT_Female_Caucasian_23_BRH600228_BNo treatment 50 Female N/A Caucasian
SA005055MaleVsFemale_NT_Female_Caucasian_23_BRH600228_ANo treatment 50 Female N/A Caucasian
SA005056MaleVsFemale_NT_Female_Caucasian_23_BRH600225_CNo treatment 50 Female N/A Caucasian
SA005057MaleVsFemale_NT_Female_Caucasian_23_BRH600225_DNo treatment 50 Female N/A Caucasian
SA005058MaleVsFemale_NT_Female_Caucasian_24_BRH600215_CNo treatment 50 Female N/A Caucasian
SA005059MaleVsFemale_NT_Female_Caucasian_24_BRH600215_DNo treatment 50 Female N/A Caucasian
SA005060MaleVsFemale_NT_Female_Caucasian_24_BRH600223_BNo treatment 50 Female N/A Caucasian
SA005061MaleVsFemale_NT_Female_Caucasian_24_BRH600217_DNo treatment 50 Female N/A Caucasian
SA005062MaleVsFemale_NT_Female_Caucasian_24_BRH600229_ANo treatment 50 Female N/A Caucasian
SA005063MaleVsFemale_NT_Female_Caucasian_24_BRH600229_BNo treatment 50 Female N/A Caucasian
SA005064MaleVsFemale_NT_Female_Caucasian_24_BRH600217_CNo treatment 50 Female N/A Caucasian
SA005065MaleVsFemale_NT_Female_Caucasian_23_BRH600224_DNo treatment 50 Female N/A Caucasian
SA005066MaleVsFemale_NT_Female_Caucasian_24_BRH600223_ANo treatment 50 Female N/A Caucasian
SA005067MaleVsFemale_NT_Female_Caucasian_23_BRH600218_ANo treatment 50 Female N/A Caucasian
SA005068MaleVsFemale_NT_Female_Caucasian_23_BRH600224_CNo treatment 50 Female N/A Caucasian
SA005069MaleVsFemale_NT_Female_Caucasian_22_BRH600221_DNo treatment 50 Female N/A Caucasian
SA005070MaleVsFemale_NT_Female_Caucasian_22_BRH600221_CNo treatment 50 Female N/A Caucasian
SA005071MaleVsFemale_NT_Female_Caucasian_23_BRH600213_DNo treatment 50 Female N/A Caucasian
SA005072MaleVsFemale_NT_Female_Caucasian_23_BRH600213_CNo treatment 50 Female N/A Caucasian
SA005073MaleVsFemale_NT_Female_Caucasian_23_BRH600218_BNo treatment 50 Female N/A Caucasian
SA005074MaleVsFemale_NT_Female_Caucasian_23_BRH600214_BNo treatment 50 Female N/A Caucasian
SA005075MaleVsFemale_NT_Female_Caucasian_23_BRH600214_ANo treatment 50 Female N/A Caucasian
SA005076MaleVsFemale_NT_Female_Hispanic_23_BRH600210_BNo treatment 50 Female N/A Hispanic
SA005077MaleVsFemale_NT_Female_Hispanic_23_BRH600212_DNo treatment 50 Female N/A Hispanic
SA005078MaleVsFemale_NT_Female_Hispanic_24_BRH600220_ANo treatment 50 Female N/A Hispanic
SA005079MaleVsFemale_NT_Female_Hispanic_24_BRH600222_DNo treatment 50 Female N/A Hispanic
SA005080MaleVsFemale_NT_Female_Hispanic_23_BRH600212_CNo treatment 50 Female N/A Hispanic
SA005081MaleVsFemale_NT_Female_Hispanic_24_BRH600220_BNo treatment 50 Female N/A Hispanic
SA005082MaleVsFemale_NT_Female_Hispanic_24_BRH600222_CNo treatment 50 Female N/A Hispanic
SA005083MaleVsFemale_NT_Female_Hispanic_23_BRH600210_ANo treatment 50 Female N/A Hispanic
SA005084MaleVsFemale_NT_Male_Caucasian_21_BRH600204_DNo treatment 50 Male N/A Caucasian
SA005085MaleVsFemale_NT_Male_Caucasian_22_BRH600190_ANo treatment 50 Male N/A Caucasian
SA005086MaleVsFemale_NT_Male_Caucasian_21_BRH600204_CNo treatment 50 Male N/A Caucasian
SA005087MaleVsFemale_NT_Male_Caucasian_21_BRH600202_DNo treatment 50 Male N/A Caucasian
SA005088MaleVsFemale_NT_Male_Caucasian_21_BRH600202_CNo treatment 50 Male N/A Caucasian
SA005089MaleVsFemale_NT_Male_Caucasian_22_BRH600190_BNo treatment 50 Male N/A Caucasian
SA005090MaleVsFemale_NT_Male_Caucasian_22_BRH600194_DNo treatment 50 Male N/A Caucasian
SA005091MaleVsFemale_NT_Male_Caucasian_22_BRH600199_CNo treatment 50 Male N/A Caucasian
SA005092MaleVsFemale_NT_Male_Caucasian_22_BRH600199_DNo treatment 50 Male N/A Caucasian
SA005093MaleVsFemale_NT_Male_Caucasian_22_BRH600198_BNo treatment 50 Male N/A Caucasian
SA005094MaleVsFemale_NT_Male_Caucasian_22_BRH600198_ANo treatment 50 Male N/A Caucasian
SA005095MaleVsFemale_NT_Male_Caucasian_21_BRH600201_ANo treatment 50 Male N/A Caucasian
SA005096MaleVsFemale_NT_Male_Caucasian_22_BRH600194_CNo treatment 50 Male N/A Caucasian
SA005097MaleVsFemale_NT_Male_Caucasian_21_BRH600201_BNo treatment 50 Male N/A Caucasian
SA005098MaleVsFemale_NT_Male_Caucasian_20_BRH600193_BNo treatment 50 Male N/A Caucasian
SA005099MaleVsFemale_NT_Male_Caucasian_20_BRH600193_ANo treatment 50 Male N/A Caucasian
SA005100MaleVsFemale_NT_Male_Caucasian_21_BRH600197_DNo treatment 50 Male N/A Caucasian
SA005101MaleVsFemale_NT_Male_Caucasian_20_BRH600196_BNo treatment 50 Male N/A Caucasian
SA005102MaleVsFemale_NT_Male_Caucasian_20_BRH600196_ANo treatment 50 Male N/A Caucasian
SA005103MaleVsFemale_NT_Male_Caucasian_21_BRH600192_ANo treatment 50 Male N/A Caucasian
SA005104MaleVsFemale_NT_Male_Caucasian_21_BRH600192_BNo treatment 50 Male N/A Caucasian
SA005105MaleVsFemale_NT_Male_Caucasian_21_BRH600197_CNo treatment 50 Male N/A Caucasian
SA005106MaleVsFemale_NT_Male_Hispanic_21_BRH600209_CNo treatment 50 Male N/A Hispanic
SA005107MaleVsFemale_NT_Male_Hispanic_21_BRH600205_BNo treatment 50 Male N/A Hispanic
SA005108MaleVsFemale_NT_Male_Hispanic_22_BRH600203_BNo treatment 50 Male N/A Hispanic
SA005109MaleVsFemale_NT_Male_Hispanic_21_BRH600191_BNo treatment 50 Male N/A Hispanic
SA005110MaleVsFemale_NT_Male_Hispanic_21_BRH600209_DNo treatment 50 Male N/A Hispanic
SA005111MaleVsFemale_NT_Male_Hispanic_22_BRH600195_CNo treatment 50 Male N/A Hispanic
SA005112MaleVsFemale_NT_Male_Hispanic_21_BRH600191_ANo treatment 50 Male N/A Hispanic
SA005113MaleVsFemale_NT_Male_Hispanic_22_BRH600195_DNo treatment 50 Male N/A Hispanic
SA005114MaleVsFemale_NT_Male_Hispanic_22_BRH600200_ANo treatment 50 Male N/A Hispanic
SA005115MaleVsFemale_NT_Male_Hispanic_22_BRH600200_BNo treatment 50 Male N/A Hispanic
SA005116MaleVsFemale_NT_Male_Hispanic_22_BRH600203_ANo treatment 50 Male N/A Hispanic
SA005117MaleVsFemale_NT_Male_Hispanic_21_BRH600205_ANo treatment 50 Male N/A Hispanic
SA005118MaleVsFemale_NT_Male_Hispanic_20_BRH600206_CNo treatment 50 Male N/A Hispanic
SA005119MaleVsFemale_NT_Male_Hispanic_20_BRH600208_DNo treatment 50 Male N/A Hispanic
Showing page 1 of 3     Results:    1  2  3  Next     Showing results 1 to 100 of 235
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