Summary of study ST000096

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000079. The data can be accessed directly via it's Project DOI: 10.21228/M82S3K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000096
Study TitleA study of changes in lipid metabolism of ovarian cancer cells co-cultured with adipocytes: UPLC-QTRAP MS analysis
Study TypeTimecourse
Study SummaryThe study investigated the interaction between omental adipocytes and OvCa cells, as a follow up to preliminary data indicating this leads to reprograming of metabolic (especially lipid) profiles in both adipocytes and OvCa cells as ovarian cancer cells (OvCa) readily metastasize to the omental fat pad in the abdomen and stimulate the release of fatty acids. In order to mimic the interaction between OvCa and omental adipocytes during metastasis, a coculture system was used that employed OvCa cells and primary human adipocytes isolated from omentum. Human primary adipocytes were isolated from omental explants from patients undergoing surgery for benign conditions. After surgical removal, omental tissue was digested with collagenase I, and primary cultures of adipocytes were established, characterized, and incorporated into the co-culture. The primary adipocytes were isolated and co-cultured with the OvCa cell line Skov3ip1. In this current submission, the the samples will be collected at 4, 18 and 24 hour time points post co-culture to determine the time dependent effect on lipid mediators, including oxylipins and ceramides. The study results included in this DRCC submission were the 18 hour time point data for oxylipins and ceramides from targeted metabolomic analysis of lipid mediators performed by the Newman lab.
Institute
University of California, Davis
DepartmentU.S.D.A. Western Human Nutrition Research Center
LaboratoryNewman
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2014-07-24
Num Groups3
Total Subjects21
Raw Data AvailableYes
Raw Data File Type(s)mzML
Uploaded File Size30 M
Analysis Type DetailLC-MS
Release Date2015-02-03
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M82S3K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type Timepoint
SA005411S33AAdipocyte 18 hours
SA005412S35AAdipocyte 18 hours
SA005413S32AAdipocyte 18 hours
SA005414S34AAdipocyte 18 hours
SA005415S29AAdipocyte 18 hours
SA005416S30AAdipocyte 18 hours
SA005417S31AAdipocyte 18 hours
SA005418S17AAdipocyte 4 hours
SA005419S25AAdipocyte 4 hours
SA005420S19AAdipocyte 4 hours
SA005421S24AAdipocyte 4 hours
SA005422S26AAdipocyte 4 hours
SA005423S23AAdipocyte 4 hours
SA005424S20AAdipocyte 4 hours
SA005425S34CCo-culture 18 hours
SA005426S32CCo-culture 18 hours
SA005427S33CCo-culture 18 hours
SA005428S31CCo-culture 18 hours
SA005429S30CCo-culture 18 hours
SA005430S29CCo-culture 18 hours
SA005431S35CCo-culture 18 hours
SA005432S17CCo-culture 4 hours
SA005433S24CCo-culture 4 hours
SA005434S25CCo-culture 4 hours
SA005435S23CCo-culture 4 hours
SA005436S20CCo-culture 4 hours
SA005437S19CCo-culture 4 hours
SA005438S26CCo-culture 4 hours
SA005439S35BControl 18 hours
SA005440S31BControl 18 hours
SA005441S30BControl 18 hours
SA005442S32BControl 18 hours
SA005443S29BControl 18 hours
SA005444S33BControl 18 hours
SA005445S34BControl 18 hours
SA005446S24BControl 4 hours
SA005447S26BControl 4 hours
SA005448S23BControl 4 hours
SA005449S25BControl 4 hours
SA005450S19BControl 4 hours
SA005451S20BControl 4 hours
SA005452S17BControl 4 hours
SA005453Media blankMedia blank 4 hours
SA005454Blank mediaMedia blank NA
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