Summary of Study ST000147
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000147 |
| Study Title | 13C targeted metabolomics |
| Study Summary | Jurkat parental cells (JP6) or cells stably expressing the pCDNA Noxa vector (N5) cells were cultured to confluence and counted. 20E6 cells in triplicate were resuspended in glucose free or glutamine free medium for 2 hours. Cells were then pelleted and resuspended in 13C-1,2 Glucose (10mM) or 13C-U-glutamine (4mM) for 24 hours. Cells were pelleted and spent medium was collected. Cell pellets were washed 1X with ice cold PBS and cell pellets were resuspended in 400ul -20 methanol. Cells and media were snap frozen in liquid nitrogen and stored at -80. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-03-13 |
| Num Groups | 8 |
| Total Subjects | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 2.6 G |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2015-03-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment | Cell type | Harvest |
|---|---|---|---|---|
| SA008061 | S00015033 | 13C glucose labeled | Cloned Jurkat cell mid level Noxa | cells |
| SA008062 | S00015035 | 13C glucose labeled | Cloned Jurkat cell mid level Noxa | cells |
| SA008063 | S00015034 | 13C glucose labeled | Cloned Jurkat cell mid level Noxa | cells |
| SA008064 | S00015045 | 13C glucose labeled | Cloned Jurkat cell mid level Noxa | spent medium |
| SA008065 | S00015046 | 13C glucose labeled | Cloned Jurkat cell mid level Noxa | spent medium |
| SA008066 | S00015047 | 13C glucose labeled | Cloned Jurkat cell mid level Noxa | spent medium |
| SA008067 | S00015038 | 13C glucose labeled | Noxa overexpressed Jurkat cell | cells |
| SA008068 | S00015036 | 13C glucose labeled | Noxa overexpressed Jurkat cell | cells |
| SA008069 | S00015037 | 13C glucose labeled | Noxa overexpressed Jurkat cell | cells |
| SA008070 | S00015050 | 13C glucose labeled | Noxa overexpressed Jurkat cell | spent medium |
| SA008071 | S00015048 | 13C glucose labeled | Noxa overexpressed Jurkat cell | spent medium |
| SA008072 | S00015049 | 13C glucose labeled | Noxa overexpressed Jurkat cell | spent medium |
| SA008073 | S00015041 | 13C glutamine labeled | Cloned Jurkat cell mid level Noxa | cells |
| SA008074 | S00015039 | 13C glutamine labeled | Cloned Jurkat cell mid level Noxa | cells |
| SA008075 | S00015040 | 13C glutamine labeled | Cloned Jurkat cell mid level Noxa | cells |
| SA008076 | S00015051 | 13C glutamine labeled | Cloned Jurkat cell mid level Noxa | spent medium |
| SA008077 | S00015053 | 13C glutamine labeled | Cloned Jurkat cell mid level Noxa | spent medium |
| SA008078 | S00015052 | 13C glutamine labeled | Cloned Jurkat cell mid level Noxa | spent medium |
| SA008079 | S00015044 | 13C glutamine labeled | Noxa overexpressed Jurkat cell | cells |
| SA008080 | S00015043 | 13C glutamine labeled | Noxa overexpressed Jurkat cell | cells |
| SA008081 | S00015042 | 13C glutamine labeled | Noxa overexpressed Jurkat cell | cells |
| SA008082 | S00015056 | 13C glutamine labeled | Noxa overexpressed Jurkat cell | spent medium |
| SA008083 | S00015054 | 13C glutamine labeled | Noxa overexpressed Jurkat cell | spent medium |
| SA008084 | S00015055 | 13C glutamine labeled | Noxa overexpressed Jurkat cell | spent medium |
| Showing results 1 to 24 of 24 |
