Summary of study ST000393

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000307. The data can be accessed directly via it's Project DOI: 10.21228/M8Z01Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000393
Study TitleLong-Chain Fatty Acid Combustion Rate Is Associated with Unique Metabolite Profiles in Skeletal Muscle Mitochondria
Study SummaryIncomplete or limited long-chain fatty acid (LCFA) combustion in skeletal muscle has been associated with insulin resistance. Signals that are responsive to shifts in LCFA β-oxidation rate or degree of intramitochondrial catabolism are hypothesized to regulate second messenger systems downstream of the insulin receptor. Recent evidence supports a causal link between mitochondrial LCFA combustion in skeletal muscle and insulin resistance. We have used unbiased metabolite profiling of mouse muscle mitochondria with the aim of identifying candidate metabolites within or effluxed from mitochondria and that are shifted with LCFA combustion rate. This proof-of-principle study establishes that large-scale metabolomics methods can be applied to organelle-level models to discover metabolite patterns reflective of LCFA combustion, which may lead to identification of molecules linking muscle fat metabolism and insulin signaling. Our results suggest that future studies should focus on the fate of effluxed TCA cycle intermediates and on mechanisms ensuring their replenishment during LCFA metabolism in skeletal muscle.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2016-05-06
Publicationshttp://dx.doi.org/10.1371/journal.pone.0009834
Raw Data AvailableYes
Raw Data File Type(s).peg
Analysis Type DetailGC-MS
Release Date2016-06-18
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M8Z01Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Organ Time Dose
SA018676061215byusa1091_1cytosol 0 min palmitic acid 2.4 uM
SA018677061215byusa1127_1cytosol 0 min palmitic acid 2.4 uM
SA018678061215byusa1092_1cytosol 0 min palmitic acid 2.4 uM
SA018679061215byusa1119_1cytosol 0 min palmitic acid 2.4 uM
SA018680061215byusa1096_1cytosol 0 min palmitic acid 2.4 uM
SA018681061215byusa1088_1cytosol 0 min palmitic acid 2.4 uM
SA018682061215byusa1134_1cytosol 0 min palmitic acid 9.6 uM
SA018683061215byusa1090_1cytosol 0 min palmitic acid 9.6 uM
SA018684061215byusa1086_1cytosol 0 min palmitic acid 9.6 uM
SA018685061215byusa1103_1cytosol 0 min palmitic acid 9.6 uM
SA018686061215byusa1079_1cytosol 0 min palmitic acid 9.6 uM
SA018687061215byusa1065_1cytosol 0 min palmitic acid 9.6 uM
SA018688061215byusa1112_1cytosol 0 min palmitoyl carnitine 9.6 uM
SA018689061215byusa1075_1cytosol 0 min palmitoyl carnitine 9.6 uM
SA018690061215byusa1124_1cytosol 0 min palmitoyl carnitine 9.6 uM
SA018691061215byusa1087_1cytosol 0 min palmitoyl carnitine 9.6 uM
SA018692061215byusa1077_1cytosol 0 min palmitoyl carnitine 9.6 uM
SA018693061215byusa1114_1cytosol 0 min palmitoyl carnitine 9.6 uM
SA018694061215byusa1128_1cytosol 20 min palmitic acid 2.4 uM
SA018695061215byusa1074_1cytosol 20 min palmitic acid 2.4 uM
SA018696061215byusa1105_1cytosol 20 min palmitic acid 2.4 uM
SA018697061215byusa1129_1cytosol 20 min palmitic acid 2.4 uM
SA018698061215byusa1116_1cytosol 20 min palmitic acid 2.4 uM
SA018699061215byusa1123_1cytosol 20 min palmitic acid 2.4 uM
SA018700061215byusa1100_1cytosol 20 min palmitic acid 9.6 uM
SA018701061215byusa1080_1cytosol 20 min palmitic acid 9.6 uM
SA018702061215byusa1085_1cytosol 20 min palmitic acid 9.6 uM
SA018703061215byusa1076_1cytosol 20 min palmitic acid 9.6 uM
SA018704061215byusa1078_1cytosol 20 min palmitic acid 9.6 uM
SA018705061215byusa1093_1cytosol 20 min palmitic acid 9.6 uM
SA018706061215byusa1133_1cytosol 20 min palmitoyl carnitine 9.6 uM
SA018707061215byusa1109_1cytosol 20 min palmitoyl carnitine 9.6 uM
SA018708061215byusa1084_1cytosol 20 min palmitoyl carnitine 9.6 uM
SA018709061215byusa1068_1cytosol 20 min palmitoyl carnitine 9.6 uM
SA018710061215byusa1106_1cytosol 20 min palmitoyl carnitine 9.6 uM
SA018711061215byusa1125_1cytosol 20 min palmitoyl carnitine 9.6 uM
SA018712061215byusa1107_1mitochondria 0 min palmitic acid 2.4 uM
SA018713061215byusa1072_1mitochondria 0 min palmitic acid 2.4 uM
SA018714061215byusa1066_1mitochondria 0 min palmitic acid 2.4 uM
SA018715061215byusa1089_1mitochondria 0 min palmitic acid 2.4 uM
SA018716061215byusa1083_1mitochondria 0 min palmitic acid 2.4 uM
SA018717061215byusa1081_1mitochondria 0 min palmitic acid 2.4 uM
SA018718061215byusa1113_1mitochondria 0 min palmitic acid 9.6 uM
SA018719061215byusa1095_1mitochondria 0 min palmitic acid 9.6 uM
SA018720061215byusa1115_1mitochondria 0 min palmitic acid 9.6 uM
SA018721061215byusa1099_1mitochondria 0 min palmitic acid 9.6 uM
SA018722061215byusa1094_1mitochondria 0 min palmitic acid 9.6 uM
SA018723061215byusa1122_1mitochondria 0 min palmitoyl carnitine 9.6 uM
SA018724061215byusa1136_1mitochondria 0 min palmitoyl carnitine 9.6 uM
SA018725061215byusa1098_1mitochondria 0 min palmitoyl carnitine 9.6 uM
SA018726061215byusa1111_1mitochondria 0 min palmitoyl carnitine 9.6 uM
SA018727061215byusa1110_1mitochondria 0 min palmitoyl carnitine 9.6 uM
SA018728061215byusa1101_1mitochondria 0 min palmitoyl carnitine 9.6 uM
SA018729061215byusa1097_1mitochondria 20 min palmitic acid 2.4 uM
SA018730061215byusa1104_1mitochondria 20 min palmitic acid 2.4 uM
SA018731061215byusa1067_1mitochondria 20 min palmitic acid 2.4 uM
SA018732061215byusa1130_1mitochondria 20 min palmitic acid 2.4 uM
SA018733061215byusa1120_1mitochondria 20 min palmitic acid 2.4 uM
SA018734061215byusa1131_1mitochondria 20 min palmitic acid 9.6 uM
SA018735061215byusa1071_1mitochondria 20 min palmitic acid 9.6 uM
SA018736061215byusa1082_1mitochondria 20 min palmitic acid 9.6 uM
SA018737061215byusa1102_1mitochondria 20 min palmitic acid 9.6 uM
SA018738061215byusa1135_1mitochondria 20 min palmitic acid 9.6 uM
SA018739061215byusa1118_1mitochondria 20 min palmitoyl carnitine 9.6 uM
SA018740061215byusa1069_1mitochondria 20 min palmitoyl carnitine 9.6 uM
SA018741061215byusa1132_1mitochondria 20 min palmitoyl carnitine 9.6 uM
SA018742061215byusa1070_1mitochondria 20 min palmitoyl carnitine 9.6 uM
SA018743061215byusa1121_1mitochondria 20 min palmitoyl carnitine 9.6 uM
Showing results 1 to 68 of 68
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