Summary of Study ST000423

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000331. The data can be accessed directly via it's Project DOI: 10.21228/M80S3Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000423
Study TitleDifferences in mycoplasma growth due to different mediums
Study SummaryThe object is to learn if there are variations in the lipid profiles of the three genomic variants (relative to one another) and if there are difference the lipid profiles due to growth in medium having different supplements. Mycoplasmas are eubacteria, but have only a single plasma membrane and no cell wall. They acquire FAs and cholesterol and other (perhaps many unknown) lipids from the medium which is complex and contains mammalian serum. Various mycoplasma species have been shown to contain a wide spectrum of bacterial lipids, but the composition is unknown for this mycoplasma species. We are particularly interested in ratios of membrane lipids among our strains, in part to gain clues about differences in metabolic pathways pertinent to membrane biogenesisÍž and to predict any underlying features that could relate to the extremely different modes of cell propagation observed among these genomic constructs.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2016-07-08
Num Groups4
Total Subjects24
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M80S3Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA021537Quigley_Inj10_SA009Bone marrow macrophage
SA021538Quigley_Inj13_SA010Bone marrow macrophage
SA021539Quigley_Inj04_SA012Bone marrow macrophage
SA021540Quigley_Inj07_SA008Bone marrow macrophage
SA021541Quigley_Inj14_SA011Bone marrow macrophage
SA021542Quigley_Inj06_SA007Bone marrow macrophage
SA021543Quigley_Inj09_SA003Resident macrophage
SA021544Quigley_Inj03_SA002Resident macrophage
SA021545Quigley_Inj08_SA004Resident macrophage
SA021546Quigley_Inj11_SA005Resident macrophage
SA021547Quigley_Inj05_SA006Resident macrophage
SA021548Quigley_Inj12_SA001Resident macrophage
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