Summary of Study ST000446

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000344. The data can be accessed directly via it's Project DOI: 10.21228/M8KK69 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000446
Study TitleHeterologous expression and detection of Apratoxins in E. coli
Study TypeOrganic extraction of E coli cultures harboring apratoxin gene cluster
Study SummaryThe apratoxin gene cluster was recovered from fosmid DNA library. The gene set responsible for the biosynthesis of the polyketide backbone was introduced in E. coli BAP strain and expressed at 30C for 24 hours. Two sets of control and experimental samples with culture broth pH adjusted at 8 and 12 respectively was extracted with ethyl acetate and dried.
Institute
University of Florida
DepartmentSECIM
Last NameKallifidas
First NameDimitris
AddressMedical Sciences Building, Rm P5-26, 1600 SW Archer Rd., FL32610
Emaildkallifidas@ufl.edu
PhoneNone
Submit Date2016-07-28
Num Groups1
Total Subjects12
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Dimitris Kallifidas Dimitris Kallifidas
https://dx.doi.org/10.21228/M8KK69
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Factors:

Subject type: bacteria cells; Subject species: Escherichia coli (Factor headings shown in green)

mb_sample_id local_sample_id pH treatment
SA022652E.coli+bAprat14_pH12_212 bAprat14
SA022653E.coli+bAprat14_pH12_312 bAprat14
SA022654E.coli+bAprat14_pH1212 bAprat14
SA022655E.coli_control_pH1212 control
SA022656E.coli_control_pH12_212 control
SA022657E.coli_control_pH12_312 control
SA022658E.coli+bAprat14_pH8_38 bAprat14
SA022659E.coli+bAprat14_pH8_28 bAprat14
SA022660E.coli+bAprat14_pH88 bAprat14
SA022661E.coli_control_pH8_28 control
SA022662E.coli_control_pH88 control
SA022663E.coli_control_pH8_38 control
Showing results 1 to 12 of 12
  logo