Summary of Study ST000460

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000354. The data can be accessed directly via it's Project DOI: 10.21228/M8930Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000460
Study TitleEffect of ATG7 reconstitution in AGY571 cells
Study TypeCells are treated with vehicle or Doxycycline - to induce expression of GFP or ATG7
Study SummaryAGY571 cells engineered to express Dox-inducible GFP or ATG7 were treated with 500 ng/mL or 1 ug/mL Doxycycline for 48 hours. Assessing the effects of ATG7 reexpression in B cell lymphoma.
Institute
University of Florida
DepartmentSECIM
LaboratoryJohn Cleveland
Last NameFernandez
First NameMario
Address12902 Magnolia Drive, MRC 4-West
Emailmario.fernandez@moffitt.org
Phone813-745-5140
Submit Date2016-09-08
Num Groups5
Total Subjects15
Study CommentsGroups collected in triplicate per time point, either treated or untreated with Doxycycline.
Analysis Type DetailLC-MS
Release Date2020-07-08
Release Version1
Mario Fernandez Mario Fernandez
https://dx.doi.org/10.21228/M8930Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Dox Concentration Ectopic Gene Expression
SA023241AGY571_009Doxycycline 1 ug/mL ATG7
SA023242AGY571_007Doxycycline 1 ug/mL ATG7
SA023243AGY571_008Doxycycline 1 ug/mL ATG7
SA023244AGY571_003Doxycycline 1 ug/mL GFP (ATG7-/-)
SA023245AGY571_001Doxycycline 1 ug/mL GFP (ATG7-/-)
SA023246AGY571_002Doxycycline 1 ug/mL GFP (ATG7-/-)
SA023247AGY571_013Doxycycline 500 ng/mL ATG7
SA023248AGY571_014Doxycycline 500 ng/mL ATG7
SA023249AGY571_015Doxycycline 500 ng/mL ATG7
SA023250AGY571_012Doxycycline 500 ng/mL GFP (ATG7-/-)
SA023251AGY571_010Doxycycline 500 ng/mL GFP (ATG7-/-)
SA023252AGY571_011Doxycycline 500 ng/mL GFP (ATG7-/-)
SA023253AGY571_006Vehicle 0 ug/mL None (ATG7-/- backgound)
SA023254AGY571_005Vehicle 0 ug/mL None (ATG7-/- backgound)
SA023255AGY571_004Vehicle 0 ug/mL None (ATG7-/- backgound)
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