Summary of Study ST000679
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000480. The data can be accessed directly via it's Project DOI: 10.21228/M8102Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000679 |
| Study Title | Functional genomics study on Non Alcoholic fatty liver disease (NAFLD) - part II |
| Study Type | MS analysis |
| Study Summary | In this experiment, vector , GCKR (WT), GCKR(P446L), PPP1R3B, TM6SF2 (WT and E167K) overexpression stable HuH-7_Lok cell lines as well as Non-target, GCKR shRNA (52621), PPP1R3B shRNA (2640), TM6SF2 shRNA (382426) knockdown HuH-7_Lok cell lines are used to do the lipidomics experiments 3 X 100 mm dishes of cells were seeded (1x106 cells/dish) for each cell line and incubated at 37? C for 24 hours, then replaced medium with delipidated serum DMEM medium. After 24 hours, treated with BSA-OA (200?M) for another 24 hours before the collection of cells Add 4mL/dish 50 mM ammonium acetate wash cells and then quench cells by adding liquid nitrogen covering all the surface of dish. Store the cell samples in -80?C freezer. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 8 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
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Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Construct |
|---|---|---|
| SA038962 | S00026062 | GCKR_52621 |
| SA038963 | S00026061 | GCKR_52621 |
| SA038964 | S00026063 | GCKR_52621 |
| SA038965 | S00026066 | NT-25-9-16 |
| SA038966 | S00026065 | NT-25-9-16 |
| SA038967 | S00026064 | NT-25-9-16 |
| SA038974 | S00026070 | PNPLA3_50053 |
| SA038975 | S00026071 | PNPLA3_50053 |
| SA038976 | S00026072 | PNPLA3_50053 |
| SA038968 | S00026059 | PNPLA3(I148M) |
| SA038969 | S00026058 | PNPLA3(I148M) |
| SA038970 | S00026060 | PNPLA3(I148M) |
| SA038971 | S00026055 | PNPLA3(WT) |
| SA038972 | S00026056 | PNPLA3(WT) |
| SA038973 | S00026057 | PNPLA3(WT) |
| SA038977 | S00026069 | PPP1R3B_2640-25-9-16 |
| SA038978 | S00026067 | PPP1R3B_2640-25-9-16 |
| SA038979 | S00026068 | PPP1R3B_2640-25-9-16 |
| SA038980 | S00026074 | TM6SF2_382426 |
| SA038981 | S00026075 | TM6SF2_382426 |
| SA038982 | S00026073 | TM6SF2_382426 |
| SA038983 | S00026053 | Vector-25-9-16 |
| SA038984 | S00026054 | Vector-25-9-16 |
| SA038985 | S00026052 | Vector-25-9-16 |
| Showing results 1 to 24 of 24 |
