Summary of Study ST000680
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000480. The data can be accessed directly via it's Project DOI: 10.21228/M8102Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000680 |
| Study Title | Functional genomics study on Non Alcoholic fatty liver disease (NAFLD) - part III |
| Study Type | MS analysis |
| Study Summary | In this experiment, vector , GCKR (WT), GCKR(P446L), PPP1R3B, TM6SF2 (WT and E167K) overexpression stable HuH-7_Lok cell lines as well as Non-target, GCKR shRNA (52621), PPP1R3B shRNA (2640), TM6SF2 shRNA (382426) knockdown HuH-7_Lok cell lines are used to do the lipidomics experiments 3 X 100 mm dishes of cells were seeded (1x106 cells/dish) for each cell line and incubated at 37? C for 24 hours, then replaced medium with delipidated serum DMEM medium. After 24 hours, treated with BSA-OA (200?M) for another 24 hours before the collection of cells Add 4mL/dish 50 mM ammonium acetate wash cells and then quench cells by adding liquid nitrogen covering all the surface of dish. Store the cell samples in -80?C freezer. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
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Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Construct |
|---|---|---|
| SA038986 | S00026198 | LOC_ASO |
| SA038987 | S00026197 | LOC_ASO |
| SA038988 | S00026199 | LOC_ASO |
| SA038989 | S00026193 | Mock |
| SA038990 | S00026192 | Mock |
| SA039000 | S00026184 | pCMV6_Vector |
| SA039001 | S00026185 | pCMV6_Vector |
| SA039002 | S00026183 | pCMV6_Vector |
| SA038991 | S00026194 | Scramble |
| SA038992 | S00026196 | Scramble |
| SA038993 | S00026195 | Scramble |
| SA038994 | S00026191 | TM6SF2(E167K) |
| SA038995 | S00026189 | TM6SF2(E167K) |
| SA038996 | S00026190 | TM6SF2(E167K) |
| SA038997 | S00026186 | TM6SF2(WT) |
| SA038998 | S00026187 | TM6SF2(WT) |
| SA038999 | S00026188 | TM6SF2(WT) |
| Showing results 1 to 17 of 17 |
