Summary of Study ST001008

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000681. The data can be accessed directly via it's Project DOI: 10.21228/M8NH4G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001008
Study TitleMulti-Platform Physiologic and Metabolic Phenotyping Reveals Microbial Toxicity (part II)
Study SummaryThe gut microbiota are susceptible to modulation by environmental stimuli and therefore can serve as biological sensors. Recent evidence suggests that xenobiotics can disrupt the interaction between the microbiota and host. Here, we describe an approach that combines in vitro microbial incubation (isolated cecal contents from mice), flow cytometry, and mass spectrometry- and 1H NMR-based metabolomics to evaluate xenobiotic-induced microbial toxicity. Tempol, a stabilized free radical scavenger known to remodel the microbial community structure and function in vivo, was studied to assess its direct effects on the gut microbiota. Microbiota were isolated from mouse cecum and were exposed to tempol for 4 h under strict anaerobic condition. The flow cytometry data suggested short term exposure of the microbiota to tempol is associated with disrupted membrane physiology as well as compromised metabolic activity. Mass spectrometry and NMR metabolomics revealed that tempol exposure significantly disrupted microbial metabolic activity, specifically indicated by changes in short chain fatty acids, branched chain amino acids, amino acids, nucleotides, glucose, and oligosaccharides. In addition, a mouse study with tempol (5 days gavage) showed similar microbial physiologic and metabolic changes, indicating the in vitro approach reflected in vivo conditions. Our results, through evaluation of microbial viability, physiology and metabolism, and comparison of in vitro and in vivo exposures with tempol, suggests that physiologic and metabolic phenotyping provides unique insight into gut microbiota toxicity.
Institute
Pennsylvania State University
Last NameNichols
First NameRobert
Address650 toftrees ave Apt #108, State College, Pa 16802
Emailrgn5011@psu.edu
Phone7247662694
Submit Date2018-07-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2018-08-27
Release Version1
Robert Nichols Robert Nichols
https://dx.doi.org/10.21228/M8NH4G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Other; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA063432C1Control
SA063433C6Control
SA063434C5Control
SA063435C2Control
SA063436C3Control
SA063437C4Control
SA063438H6High Dose Tempol
SA063439H5High Dose Tempol
SA063440H2High Dose Tempol
SA063441H4High Dose Tempol
SA063442H1High Dose Tempol
SA063443H3High Dose Tempol
SA063444L5Low Dose Tempol
SA063445L6Low Dose Tempol
SA063446L4Low Dose Tempol
SA063447L3Low Dose Tempol
SA063448L1Low Dose Tempol
SA063449L2Low Dose Tempol
SA063450M5Medium Dose Tempol
SA063451M4Medium Dose Tempol
SA063452M6Medium Dose Tempol
SA063453M3Medium Dose Tempol
SA063454M1Medium Dose Tempol
SA063455M2Medium Dose Tempol
SA063456P6PH4
SA063457P5PH4
SA063458P3PH4
SA063459P1PH4
SA063460P2PH4
SA063461P4PH4
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