Summary of Study ST001033

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000691. The data can be accessed directly via it's Project DOI: 10.21228/M8C10N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001033
Study TitleDetermination of mode of action of anti-malalrial drugs using untargeted metabolomics
Study SummaryThe mode of action of a representative active compound was investigated using an unbiased metabolomics approach, which has previously been shown to reveal both novel and established modes of action of antimalarials (Creek et al 2016, DOI: 10.1128/AAC.01226-16). The active antimalarial OSM-S-313, and the inactive analogue OSM-S-291, were incubated with trophozoite stage P. falciparum parasites for five hours alongside reference compounds including atovaquone (ATV), chloroquine (CQ), dihydroartemisisin (DHA) and three PfATP4 inhibitors, MMV00073, MMV397264 and MMV390482. Metabolomics analysis of cell pellets and spent media allowed reproducible detection of diverse metabolites from a range of metabolic pathways, with the most significant OSM-S-313-induced perturbations observed within peptide, lipid and energy metabolism, suggesting a specific impact on parasite metabolism.
Institute
Monash University
Last NameCreek
First NameDarren
AddressMonash Institute of Pharmaceutical Sciences, 381 Royal Parade, Parkville, Melbourne, Victoria, 3052, Australia
Emaildarren.creek@monash.edu
Phone+61 (0) 3 9903 9249
Submit Date2018-08-13
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2018-08-27
Release Version1
Darren Creek Darren Creek
https://dx.doi.org/10.21228/M8C10N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Plasmodium falciparum (Factor headings shown in green)

mb_sample_id local_sample_id drug treatment
SA069162P_ATV_3Atovaquone
SA069163P_ATV_1Atovaquone
SA069164P_ATV_2Atovaquone
SA069165P_ATV_4Atovaquone
SA069166P_CQ_2Chloroquine
SA069167P_CQ_4Chloroquine
SA069168P_CQ_1Chloroquine
SA069169P_CQ_3Chloroquine
SA069174P_DHA_2Dihydroartemisisin
SA069175P_DHA_4Dihydroartemisisin
SA069176P_DHA_1Dihydroartemisisin
SA069177P_DHA_3Dihydroartemisisin
SA069170P_DMSO_1DMSO
SA069171P_DMSO_3DMSO
SA069172P_DMSO_2DMSO
SA069173P_DMSO_4DMSO
SA069178P_000073_2MMV00073
SA069179P_000073_4MMV00073
SA069180P_000073_3MMV00073
SA069181P_000073_1MMV00073
SA069182P_390482_3MMV390482
SA069183P_390482_4MMV390482
SA069184P_390482_2MMV390482
SA069185P_390482_1MMV390482
SA069186P_397264_3MMV397264
SA069187P_397264_4MMV397264
SA069188P_397264_1MMV397264
SA069189P_397264_2MMV397264
SA069190P_S291_3OSM-S-291
SA069191P_S291_4OSM-S-291
SA069192P_S291_2OSM-S-291
SA069193P_S291_1OSM-S-291
SA069194P_S313_4OSM-S-313
SA069195P_S313_2OSM-S-313
SA069196P_S313_1OSM-S-313
SA069197P_S313_3OSM-S-313
Showing results 1 to 36 of 36
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