Summary of Study ST001056

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000709. The data can be accessed directly via it's Project DOI: 10.21228/M81M4X This work is supported by NIH grant, U2C- DK119886.

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Study IDST001056
Study TitleEvaluation of the short-term effects of the allelochemical umbelliferone on Triticum durum L. metabolism through GC-MS based untargeted metabolomics
Study SummaryAllelopathy is a plant defense mechanism by which they protect themselves from competitive species using specialized biochemicals in the form of secretion or volatiles released to the environment. Though, umbelliferone is a well-known allelochemical, its mechanism of action in a short-term treatment is far from established. We used ≈ 10–days old wheat seedlings treated with104 µM umbelliferone over a time course experiment covering 6 times points, i.e., 0h, 6h, 12h, 24h, 48h, and 96h and compared the metabolomic changes to control (mock-treated) plants. Using gas chromatography mass-spectrometry (GC-MS) based metabolomics efforts, we collectively obtained quantitative data on 177 metabolites that were derivatized (either derivatized singly or multiple times) or not representing 139 non-redundant (unique) metabolites. Out of these 139 metabolites, 118 were associated with a unique HMDB identifier, while 113 were associated with a KEGG identifier. Relative quantification of these metabolites across the time-course of umbelliferone treatment, revealed 22 compounds (sugars, fatty acids, secondary metabolites, organic acids, and amino acids) that changed significantly (repeated measures ANOVA, P-value < 0.05) with time. Using multivariate partial least squares discriminant analysis (PLS-DA) we showed the grouping of samples based on time-course across the control and umbelliferone treated plants, whereas the metabolite-metabolite Pearson correlation revealed tightly formed clusters of umbelliferone-derived metabolites, fatty acids, amino acids, and carbohydrates. Also, time-course of umbelliferone treatment revealed, that phospho-L-serine, maltose, and dehydroquinic acid were the top three metabolites showing highest importance in discrimination among the time-points. The above indicate a system-wide changes induced by umbelliferone, through dysregulation of primary as well as specialized metabolism.
Institute
Università Mediterranea di Reggio Calabria
DepartmentDipartimento AGRARIA
Last NameAraniti
First NameFabrizio
AddressDepartment AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, SNC I-89124, Reggio Calabria RC, Italy
Emailfabrizio.araniti@unirc.it
Phone+39-09651694283
Submit Date2018-09-12
Num Groups3
PublicationsIn process
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2020-01-13
Release Version1
Fabrizio Araniti Fabrizio Araniti
https://dx.doi.org/10.21228/M81M4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Triticum durum (Factor headings shown in green)

mb_sample_id local_sample_id Time
SA093741RT-
SA093742m/z-
SA093743T40h
SA093744T10h
SA093745T20h
SA093746T30h
SA09374712HKR412h
SA09374812HKR512h
SA09374912HKR212h
SA09375012HKR112h
SA09375112HUKR412h
SA09375212HUKR512h
SA09375312HUKR312h
SA09375412HUKR112h
SA09375512HKR312h
SA09375612HUKR212h
SA09375724HKR224h
SA09375824HKR424h
SA09375924HKR324h
SA09376024HKR124h
SA09376124HUKR324h
SA09376224HUKR124h
SA09376324HUKR224h
SA09376424HKR524h
SA09376524HUKR424h
SA09376624HUKR524h
SA09376748HUKR348h
SA09376848HUKR248h
SA09376948HUKR148h
SA09377048HKR548h
SA09377148HUKR448h
SA09377248HUKR548h
SA09377348HKR248h
SA09377448HKR348h
SA09377548HKR448h
SA09377648HKR148h
SA0937776HUKR16h
SA0937786HUKR26h
SA0937796HUKR36h
SA0937806HUKR56h
SA0937816HKR56h
SA0937826HUKR46h
SA0937836HKR46h
SA0937846HKR16h
SA0937856HKR36h
SA0937866HKR26h
SA09378796HKR496h
SA09378896HKR396h
SA09378996HKR596h
SA09379096HUKR596h
SA09379196HUKR296h
SA09379296HUKR196h
SA09379396HUKR396h
SA09379496HUKR496h
SA09379596HKR196h
SA09379696HKR296h
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