Summary of Study ST001148

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000767. The data can be accessed directly via it's Project DOI: 10.21228/M8J68Z This work is supported by NIH grant, U2C- DK119886.


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Study IDST001148
Study TitleEffect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts
Study TypeMethod
Study SummaryHuman skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility.
Mayo Clinic
Last NameWilkins
First NameJordan
Address200 First St SW
Submit Date2019-02-27
Analysis Type DetailGC/LC-MS
Release Date2019-09-23
Release Version1
Jordan Wilkins Jordan Wilkins application/zip

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Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Harvest_technique Storage_time
SA07979410268_22Methanol_dry 2_weeks
SA07979510268_23Methanol_dry 2_weeks
SA07979610268_24Methanol_dry 2_weeks
SA07979710268_12Methanol_dry 48_hours
SA07979810268_10Methanol_dry 48_hours
SA07979910268_11Methanol_dry 48_hours
SA07980010268_34Methanol_dry 4_weeks
SA07980110268_36Methanol_dry 4_weeks
SA07980210268_35Methanol_dry 4_weeks
SA07978510268_21Methanol 2_weeks
SA07978610268_20Methanol 2_weeks
SA07978710268_19Methanol 2_weeks
SA07978810268_8Methanol 48_hours
SA07978910268_7Methanol 48_hours
SA07979010268_9Methanol 48_hours
SA07979110268_33Methanol 4_weeks
SA07979210268_32Methanol 4_weeks
SA07979310268_31Methanol 4_weeks
SA07980310268_18Scraping 2_weeks
SA07980410268_17Scraping 2_weeks
SA07980510268_16Scraping 2_weeks
SA07980610268_6Scraping 48_hours
SA07980710268_5Scraping 48_hours
SA07980810268_4Scraping 48_hours
SA07980910268_28Scraping 4_weeks
SA07981010268_30Scraping 4_weeks
SA07981110268_29Scraping 4_weeks
SA07981210268_13Trypsin 2_weeks
SA07981310268_15Trypsin 2_weeks
SA07981410268_14Trypsin 2_weeks
SA07981510268_2Trypsin 48_hours
SA07981610268_3Trypsin 48_hours
SA07981710268_1Trypsin 48_hours
SA07981810268_25Trypsin 4_weeks
SA07981910268_26Trypsin 4_weeks
SA07982010268_27Trypsin 4_weeks
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