Summary of Study ST001198

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000807. The data can be accessed directly via it's Project DOI: 10.21228/M8CD73 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001198
Study TitleTargeted LC-MS/MS Analysis of Soluble Metabolites in the MeOH:H2O Phase (part-IV)
Study SummaryCyanobacteria are a model photoautotroph and a chassis for the sustainable production of fuels and chemicals. Yet, knowledge of photoautotrophic metabolism in the natural environment of day/night cycles is lacking yet has implications for improved yield from plants, algae, and cyanobacteria. Here, a thorough approach to characterizing diverse metabolites—including carbohydrates, lipids, amino acids, pigments, co-factors, nucleic acids and polysaccharides—in the model cyanobacterium Synechocystis sp. PCC 6803 (S. 6803) under sinusoidal diurnal light-dark cycles was developed and applied. A custom photobioreactor and novel multi-platform mass spectrometry workflow enabled metabolite profiling every 30-120 minutes across a 24-hour diurnal sinusoidal LD (“sinLD”) cycle peaking at 1,600 mol photons m 2 s-1. We report widespread oscillations across the sinLD cycle with 90%, 94%, and 40% of the identified polar/semi-polar, non-polar, and polymeric metabolites displaying statistically significant oscillations, respectively. Microbial growth displayed distinct lag, biomass accumulation, and cell division phases of growth. During the lag phase, amino acids (AA) and nucleic acids (NA) accumulated to high levels per cell followed by decreased levels during the biomass accumulation phase, presumably due to protein and DNA synthesis. Insoluble carbohydrates displayed sharp oscillations per cell at the day-to-night transition. Potential bottlenecks in central carbon metabolism are highlighted. Together, this report provides a comprehensive view of photosynthetic metabolite behavior with high temporal resolution, offering insight into the impact of growth synchronization to light cycles via circadian rhythms. Incorporation into computational modeling and metabolic engineering efforts promises to improve industrially-relevant strain design.
Institute
Colorado State University
DepartmentChemical and Biological Engineering
Last NamePeebles
First NameChristie
Address700 Meridian Ave, Fort Collins, CO 80523
Emailchristie.peebles@colostate.edu
Phone970-491-6779
Submit Date2019-03-02
Raw Data AvailableYes
Raw Data File Type(s)skyd
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Christie Peebles Christie Peebles
https://dx.doi.org/10.21228/M8CD73
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Factors:

Subject type: Bacteria; Subject species: Synechocystis sp. PCC 6803 (Factor headings shown in green)

mb_sample_id local_sample_id time
SA08334612-Synechocystis_6803-cell-7a-3-
SA08334711-Synechocystis_6803-cell-7a-2-
SA08334810-Synechocystis_6803-cell-7a-1-
SA0833559-Synechocystis_6803-cell-630a-3-0.5
SA0833568-Synechocystis_6803-cell-630a-2-0.5
SA0833577-Synechocystis_6803-cell-630a-1-0.5
SA08335813-Synechocystis_6803-cell-730a-10.5
SA08335915-Synechocystis_6803-cell-730a-30.5
SA08336014-Synechocystis_6803-cell-730a-20.5
SA08336118-Synechocystis_6803-cell-8a-31
SA08336216-Synechocystis_6803-cell-8a-11
SA08336317-Synechocystis_6803-cell-8a-21
SA0833526-Synechocystis_6803-cell-6a-3-1
SA0833535-Synechocystis_6803-cell-6a-2-1
SA0833544-Synechocystis_6803-cell-6a-1-1
SA08337633-Synechocystis_6803-cell-5p-310
SA08337732-Synechocystis_6803-cell-5p-210
SA08337831-Synechocystis_6803-cell-5p-110
SA08337934-Synechocystis_6803-cell-6p-111
SA08338036-Synechocystis_6803-cell-6p-311
SA08338135-Synechocystis_6803-cell-6p-211
SA08338237-Synechocystis_6803-cell-630p-111.5
SA08338338-Synechocystis_6803-cell-630p-211.5
SA08338439-Synechocystis_6803-cell-630p-311.5
SA08338541-Synechocystis_6803-cell-7p-212
SA08338642-Synechocystis_6803-cell-7p-312
SA08338740-Synechocystis_6803-cell-7p-112
SA08338844-Synechocystis_6803-cell-730p-212.5
SA08338943-Synechocystis_6803-cell-730p-112.5
SA08339045-Synechocystis_6803-cell-730p-312.5
SA08339148-Synechocystis_6803-cell-8p-313
SA08339247-Synechocystis_6803-cell-8p-213
SA08339346-Synechocystis_6803-cell-8p-113
SA08339449-Synechocystis_6803-cell-9p-114
SA08339551-Synechocystis_6803-cell-9p-314
SA08339650-Synechocystis_6803-cell-9p-214
SA08339753-Synechocystis_6803-cell-11p-216
SA08339852-Synechocystis_6803-cell-11p-116
SA08339954-Synechocystis_6803-cell-11p-316
SA08340057-Synechocystis_6803-cell-1a-318
SA08340155-Synechocystis_6803-cell-1a-118
SA08340256-Synechocystis_6803-cell-1a-218
SA08336420-Synechocystis_6803-cell-9a-22
SA08336519-Synechocystis_6803-cell-9a-12
SA08336621-Synechocystis_6803-cell-9a-32
SA0833491-Synechocystis_6803-cell-5a-1-2
SA0833502-Synechocystis_6803-cell-5a-2-2
SA0833513-Synechocystis_6803-cell-5a-3-2
SA08340360-Synechocystis_6803-cell-3a-320
SA08340458-Synechocystis_6803-cell-3a-120
SA08340559-Synechocystis_6803-cell-3a-220
SA08340661-Synechocystis_6803-cell-5a_day2-122
SA08340762-Synechocystis_6803-cell-5a_day2-222
SA08340863-Synechocystis_6803-cell-5a_day2-322
SA08340964-Synechocystis_6803-cell-6a_day2-123
SA08341065-Synechocystis_6803-cell-6a_day3-223
SA08341166-Synechocystis_6803-cell-6a_day4-323
SA08341267-Synechocystis_6803-cell-630a_day2-123.5
SA08341368-Synechocystis_6803-cell-630a_day3-223.5
SA08341469-Synechocystis_6803-cell-630a_day4-323.5
SA08341571-Synechocystis_6803-cell-7a_day2-224
SA08341672-Synechocystis_6803-cell-7a_day2-324
SA08341770-Synechocystis_6803-cell-7a_day2-124
SA083418QC-1-6426
SA083419QC-1-7126
SA083420QC-1-78b26
SA083421QC-1-826
SA083422QC-1-5726
SA083423QC-1-7826
SA083424QC-1-5026
SA083425QC-1-1526
SA083426QC-1-126
SA083427QC-1-2226
SA083428QC-1-2926
SA083429QC-1-3626
SA083430QC-1-4326
SA08336723-Synechocystis_6803-cell-11a-24
SA08336822-Synechocystis_6803-cell-11a-14
SA08336924-Synechocystis_6803-cell-11a-34
SA08337026-Synechocystis_6803-cell-1p-26
SA08337125-Synechocystis_6803-cell-1p-16
SA08337227-Synechocystis_6803-cell-1p-36
SA08337328-Synechocystis_6803-cell-3p-18
SA08337429-Synechocystis_6803-cell-3p-28
SA08337530-Synechocystis_6803-cell-3p-38
Showing results 1 to 85 of 85
  logo