Summary of Study ST001288

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000870. The data can be accessed directly via it's Project DOI: 10.21228/M8796F This work is supported by NIH grant, U2C- DK119886.

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Study IDST001288
Study TitleSubcellular organelle lipidomics in TLR-4-activated macrophages
Study SummaryLipids orchestrate biological processes by acting remotely as signaling molecules or locally as membrane components that modulate protein function. Detailed insight into lipid function requires knowledge of the subcellular localization of individual lipids. We report an analysis of the subcellular lipidome of the mammalian macrophage, a cell type that plays key roles in inflammation, immune responses, and phagocytosis. Nuclei, mitochondria, endoplasmic reticulum (ER), plasmalemma, and cytoplasm were isolated from RAW 264.7 macrophages in basal and activated states. Subsequent lipidomic analyses of major membrane lipid categories identified 229 individual/isobaric species, including 163 glycerophospholipids, 48 sphingolipids, 13 sterols, and 5 prenols. Major subcellular compartments exhibited substantially divergent glycerophospholipid profiles. Activation of macrophages by the Toll-like receptor 4-specific lipopolysaccharide Kdo2-lipid A caused significant remodeling of the subcellular lipidome. Some changes in lipid composition occurred in all compartments (e.g. increases in the levels of ceramides and the cholesterol precursors desmosterol and lanosterol). Other changes were manifest in specific organelles. For example, oxidized sterols increased and unsaturated cardiolipins decreased in mitochondria, whereas unsaturated ether-linked phosphatidylethanolamines decreased in the ER. We speculate that these changes may reflect mitochondrial oxidative stress and the release of arachidonic acid from the ER in response to cell activation.
Institute
LIPID MAPS
DepartmentMultiple
LaboratoryMultiple
Last NameFahy
First NameEoin
Address9500 Gilman, La Jolla, CA, 92093, USA
Emailefahy@ucsd.edu
Phone858-534-4076
Submit Date2019-12-17
PublicationsAndreyev AY, Fahy E, Guan Z, Kelly S, Li X, McDonald JG, Milne S, Myers D, Park H, Ryan A, Thompson BM, Wang E, Zhao Y, Brown HA, Merrill AH, Raetz CR, Russell DW, Subramaniam S, Dennis EA. Subcellular organelle lipidomics in TLR-4-activated macrophages. J Lipid Res. 2010 Sep;51(9):2785-97. doi: 10.1194/jlr.M008748. Epub 2010 Jun 23. PMID: 20574076; PMCID: PMC2918461.
Analysis Type DetailLC-MS
Release Date2020-01-22
Release Version1
Eoin Fahy Eoin Fahy
https://dx.doi.org/10.21228/M8796F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Fraction Treatment
SA093638S32Cytosol Control
SA093639S31Cytosol Control
SA093640S33Cytosol Control
SA093641S36Cytosol KLA
SA093642S34Cytosol KLA
SA093643S35Cytosol KLA
SA093644S25Dense microsomes Control
SA093645S26Dense microsomes Control
SA093646S27Dense microsomes Control
SA093647S29Dense microsomes KLA
SA093648S30Dense microsomes KLA
SA093649S28Dense microsomes KLA
SA093650S15ER Control
SA093651S14ER Control
SA093652S13ER Control
SA093653S18ER KLA
SA093654S17ER KLA
SA093655S16ER KLA
SA093656S9Mitochondria Control
SA093657S8Mitochondria Control
SA093658S7Mitochondria Control
SA093659S11Mitochondria KLA
SA093660S10Mitochondria KLA
SA093661S12Mitochondria KLA
SA093662S1Nucleus Control
SA093663S3Nucleus Control
SA093664S2Nucleus Control
SA093665S5Nucleus KLA
SA093666S6Nucleus KLA
SA093667S4Nucleus KLA
SA093668S20Plasma membrane Control
SA093669S19Plasma membrane Control
SA093670S21Plasma membrane Control
SA093671S22Plasma membrane KLA
SA093672S23Plasma membrane KLA
SA093673S24Plasma membrane KLA
SA093674S39Whole cells Control
SA093675S38Whole cells Control
SA093676S37Whole cells Control
SA093677S42Whole cells KLA
SA093678S41Whole cells KLA
SA093679S40Whole cells KLA
Showing results 1 to 42 of 42
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