Summary of Study ST001298

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000879. The data can be accessed directly via it's Project DOI: 10.21228/M82M47 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001298
Study TitleMetabolome Profiling of Synechococcus elongatus PCC 11801 strains engineered for Succinate Production
Study TypeMeasurement of relative metabolite pools of wild type and engineered Synechococcus elongatus PCC 11801 strains using Isotopic Ratio Method
Study SummaryExperiments to measure relative metabolite pools of wild type Synechococcus elongatus PCC 11801 and its recombinants producing succinate. The wild type and the engineered strains producing succinate were cultivated at 1% CO2 and their metabolome data was collected in three biological and three technical replicates (n=9). The study aims to find metabolomics changes between the wild type and the engineered to identify potential rate-limiting steps that be used as targets for improved production.
Institute
Indian Institute of Technology Bombay
DepartmentDepartment of Chemical Engineering
LaboratoryBio systems Engineering Lab
Last NameWangikar
First NamePramod P
AddressDepartment of Chemical Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India - 400076
Emailwangikar@iitb.ac.in
Phone22254215
Submit Date2020-01-03
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2020-07-05
Release Version1
Pramod P Wangikar Pramod P Wangikar
https://dx.doi.org/10.21228/M82M47
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Synechococcus elongatus PCC 11801 (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA094196SA3 BR3-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094197SA3 BR3-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094198SA3 BR2-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094199SA3 BR3-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094200SA3 BR2-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094201SA3 BR2-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094202SA3 BR1-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094203SA3 BR1-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094204SA3 BR1-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR
SA094205SA7 BR2-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094206SA7 BR1-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094207SA7 BR1-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094208SA7 BR2-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094209SA7 BR3-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094210SA7 BR3-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094211SA7 BR2-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094212SA7 BR1-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094213SA7 BR3-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD Pcpcb300 PEPC SmR , DDOP62_03790 Prbcl400 gltA Prbcl400 SBPase/FBPase KanR
SA094214SA2 BR1-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094215SA2 BR1-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094216SA2 BR3-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094217SA2 BR3-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094218SA2 BR3-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094219SA2 BR2-3△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094220SA2 BR2-1△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094221SA2 BR2-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094222SA2 BR1-2△DOP62_03525 PpsbaI OgdA PpsbaIII SsaD SmR
SA094178WT EP BR2-2Wild type
SA094179WT EP BR2-3Wild type
SA094180WT EP BR3-1Wild type
SA094181WT EP BR2-1Wild type
SA094182WT EP BR1-3Wild type
SA094183WT BR1-1Wild type
SA094184WT EP BR1-1Wild type
SA094185WT EP BR1-2Wild type
SA094186WT EP BR3-3Wild type
SA094187WT EP BR3-2Wild type
SA094188WT BR2-1Wild type
SA094189WT BR1-3Wild type
SA094190WT BR1-2Wild type
SA094191WT BR2-3Wild type
SA094192WT BR2-2Wild type
SA094193WT BR3-3Wild type
SA094194WT BR3-2Wild type
SA094195WT BR3-1Wild type
Showing results 1 to 45 of 45
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