Summary of Study ST001302

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000883. The data can be accessed directly via it's Project DOI: 10.21228/M8JM4K This work is supported by NIH grant, U2C- DK119886.

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Study IDST001302
Study TitleMetabolome Profiling of a Fast-growing Cyanobacterium Synechococcus elongatus PCC 11801 under Diurnal Cycle
Study TypeMeasurement of relative metabolite pools under diurnal cycle using isotopic ratio method
Study SummaryExperiments were carried out by growing Synechococcus elongatus PCC 11801 cells in multicultivators designed to provide sinusoidal light. The period was 14:10 (light-dark). The samples for metabolomics analysis were collected in the second diurnal cycle at an interval of 6 hours starting from 25th hour (25, 31, 37 and 43 hours). The light intensity amplitude was 600 µmole photons.m-2. s-1. Additionally samples were also collected using cells grown under continuous light illumination of 600 µmole photons.m-2. s-1 to compare the difference in metabolite levels compared to that in the diurnal cycle.
Institute
Indian Institute of Technology Bombay
DepartmentDepartment of Chemical Engineering
LaboratoryBiosystems Engineering Lab
Last NameWangikar
First NamePramod P
AddressDepartment of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, India - 400076
Emailwangikar@iitb.ac.in
Phone+91 (22) 2576 7232 (o)
Submit Date2020-01-06
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2020-10-11
Release Version1
Pramod P Wangikar Pramod P Wangikar
https://dx.doi.org/10.21228/M8JM4K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Synechococcus elongatus PCC 11801 (Factor headings shown in green)

mb_sample_id local_sample_id Time (h) Treatment Condition
SA09429411801 LDC 25-HR BR2 IS IDA-125 MORNING
SA09429511801 LDC 25-HR BR1 IS IDA-325 MORNING
SA09429611801 LDC 25-HR BR1 IS IDA-225 MORNING
SA09429711801 LDC 25-HR BR1 IS IDA-125 MORNING
SA09429811801 LDC 25-HR BR2 IS IDA-225 MORNING
SA09429911801 LDC 25-HR BR3 IS IDA-225 MORNING
SA09430011801 LDC 25-HR BR3 IS IDA-125 MORNING
SA09430111801 LDC 25-HR BR2 IS IDA-325 MORNING
SA09430211801 LDC 25-HR BR3 IS IDA-325 MORNING
SA09430311801 LDC 31-HR BR2 IS IDA-231 MID-DAY
SA09430411801 LDC 31-HR BR1 IS IDA-331 MID-DAY
SA09430511801 LDC 31-HR BR1 IS IDA-231 MID-DAY
SA09430611801 LDC 31-HR BR1 IS IDA-131 MID-DAY
SA09430711801 LDC 31-HR BR2 IS IDA-131 MID-DAY
SA09430811801 LDC 31-HR BR2 IS IDA-331 MID-DAY
SA09430911801 LDC 31-HR BR3 IS IDA-231 MID-DAY
SA09431011801 LDC 31-HR BR3 IS IDA-131 MID-DAY
SA09431111801 LDC 31-HR BR3 IS IDA-331 MID-DAY
SA09431211801 LDC 37-HR BR2 IS IDA-237 EVENING
SA09431311801 LDC 37-HR BR2 IS IDA-337 EVENING
SA09431411801 LDC 37-HR BR3 IS IDA-137 EVENING
SA09431511801 LDC 37-HR BR3 IS IDA-237 EVENING
SA09431611801 LDC 37-HR BR2 IS IDA-137 EVENING
SA09431711801 LDC 37-HR BR1 IS IDA-337 EVENING
SA09431811801 LDC 37-HR BR1 IS IDA-137 EVENING
SA09431911801 LDC 37-HR BR1 IS IDA-237 EVENING
SA09432011801 LDC 37-HR BR3 IS IDA-337 EVENING
SA09432111801 LDC 43-HR BR1 IS IDA-143 MID-NIGHT
SA09432211801 LDC 43-HR BR2 IS IDA-343 MID-NIGHT
SA09432311801 LDC 43-HR BR2 IS IDA-243 MID-NIGHT
SA09432411801 LDC 43-HR BR2 IS IDA-143 MID-NIGHT
SA09432511801 LDC 43-HR BR3 IS IDA-143 MID-NIGHT
SA09432611801 LDC 43-HR BR3 IS IDA-243 MID-NIGHT
SA09432711801 LDC 43-HR BR1 IS IDA-243 MID-NIGHT
SA09432811801 LDC 43-HR BR3 IS IDA-343 MID-NIGHT
SA09432911801 LDC 43-HR BR1 IS IDA-343 MID-NIGHT
SA09428511801 600uE CLDC BR2 IS IDA-2- CONTINUOUS LIGHT ILLUMINATION
SA09428611801 600uE CLDC BR2 IS IDA-1- CONTINUOUS LIGHT ILLUMINATION
SA09428711801 600uE CLDC BR1 IS IDA-3- CONTINUOUS LIGHT ILLUMINATION
SA09428811801 600uE CLDC BR1 IS IDA-1- CONTINUOUS LIGHT ILLUMINATION
SA09428911801 600uE CLDC BR2 IS IDA-3- CONTINUOUS LIGHT ILLUMINATION
SA09429011801 600uE CLDC BR3 IS IDA-2- CONTINUOUS LIGHT ILLUMINATION
SA09429111801 600uE CLDC BR3 IS IDA-1- CONTINUOUS LIGHT ILLUMINATION
SA09429211801 600uE CLDC BR3 IS IDA-3- CONTINUOUS LIGHT ILLUMINATION
SA09429311801 600uE CLDC BR1 IS IDA-2- CONTINUOUS LIGHT ILLUMINATION
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