Summary of Study ST001393
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000956. The data can be accessed directly via it's Project DOI: 10.21228/M84386 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001393 |
| Study Title | Sea-ice diatom compatible solute shifts |
| Study Type | Compatible solutes were quantified in sea-ice diatoms |
| Study Summary | Sea-ice algae provide an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2,3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4 fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, reached the highest intracellular compatible solute concentrations of ~ 85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
| Institute | University of Washington |
| Department | School of Oceanography |
| Laboratory | Ingalls Lab |
| Last Name | Dawson |
| First Name | Hannah |
| Address | 1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA 98195 |
| hmdawson@uw.edu | |
| Phone | 2062216750 |
| Submit Date | 2020-03-24 |
| Publications | Dawson et al., Elementa |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-09-29 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Other; Subject species: Nitzschia lecointei (Factor headings shown in green)
| mb_sample_id | local_sample_id | Type | Salinity | Temp_degC |
|---|---|---|---|---|
| SA113432 | MediaBlk_ppt32 | Blk | 32 | NA |
| SA113433 | MediaBlk_ppt40 | Blk | 40 | NA |
| SA113434 | ASWFilterBlk_1 | Blk | NA | NA |
| SA113435 | ASWFilterBlk_2 | Blk | NA | NA |
| SA113436 | ASWFilterBlk_3 | Blk | NA | NA |
| SA113437 | 32ppt-1C_A | Smp | 32 | -1 |
| SA113438 | 32ppt-1C_B | Smp | 32 | -1 |
| SA113439 | 32ppt-1C_C | Smp | 32 | -1 |
| SA113440 | 32ppt4C_C | Smp | 32 | 4 |
| SA113441 | 32ppt4C_A | Smp | 32 | 4 |
| SA113442 | 32ppt4C_B | Smp | 32 | 4 |
| SA113443 | 40ppt-1C_B | Smp | 40 | -1 |
| SA113444 | 40ppt-1C_A | Smp | 40 | -1 |
| SA113445 | 40ppt-1C_C | Smp | 40 | -1 |
| SA113446 | 40ppt4C_B | Smp | 40 | 4 |
| SA113447 | 40ppt4C_C | Smp | 40 | 4 |
| SA113448 | 40ppt4C_A | Smp | 40 | 4 |
| SA113449 | S2C_6 | Smp | NA | NA |
| SA113450 | S2C_5 | Smp | NA | NA |
| SA113451 | S2C_4 | Smp | NA | NA |
| Showing results 1 to 20 of 20 |
