Summary of study ST001438

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000988. The data can be accessed directly via it's Project DOI: 10.21228/M80680 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001438
Study TitleSub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - MSC
Study SummaryThe human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. A second example to illustrate TENGi MS capabilities involves rare cell metabolomics of cultured mesenchymal stromal cells (MSCs), a cell type that has shown potential for treating a variety of chronic diseases. Examination of metabolic changes of MSCs cultured under conditions that may impact in vitro therapeutic activity, such as aggregate culture, or preconditioning with interferon gamma (IFN- γ)13, is critical for identifying attributes of cell quality. Reducing cell numbers required to perform MSC metabolomic analysis is essential for improving the manufacturing of highly therapeutic MSCs without significantly impeding production.
Institute
Georgia Institute of Technology
Last NameFernandez
First NameFacundo
Address901 Atlantic Dr NE, Atlanta, GA, 30332, USA
Emailfernandez@gatech.edu
Phone404-385-4432
Submit Date2020-08-02
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailESI
Release Date2020-09-14
Release Version1
Facundo Fernandez Facundo Fernandez
https://dx.doi.org/10.21228/M80680
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA122068Blank_3Blank
SA122069Blank_2Blank
SA122070QC_C1QC
SA122071QC_C3QC
SA122072QC_B3QC
SA122073QC_B2QC
SA122074QC_A1QC
SA122075QC_A2QC
SA122076S6_2Stimulated
SA122077S1_1Stimulated
SA122078S6_1Stimulated
SA122079S3_1Stimulated
SA122080S2_3Stimulated
SA122081S2_1Stimulated
SA122082S1_2Stimulated
SA122083S3_2Stimulated
SA122084S4_2Stimulated
SA122085S5_3Stimulated
SA122086S5_1Stimulated
SA122087S4_3Stimulated
SA122088U6_3Unstimulated
SA122089U3_1Unstimulated
SA122090U2_3Unstimulated
SA122091U2_2Unstimulated
SA122092U1_2Unstimulated
SA122093U1_1Unstimulated
SA122094U3_3Unstimulated
SA122095U4_1Unstimulated
SA122096U5_3Unstimulated
SA122097U5_2Unstimulated
SA122098U4_2Unstimulated
SA122099U6_1Unstimulated
Showing results 1 to 32 of 32
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