Summary of Study ST001438
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000988. The data can be accessed directly via it's Project DOI: 10.21228/M80680 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001438 |
| Study Title | Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - MSC |
| Study Summary | The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. A second example to illustrate TENGi MS capabilities involves rare cell metabolomics of cultured mesenchymal stromal cells (MSCs), a cell type that has shown potential for treating a variety of chronic diseases. Examination of metabolic changes of MSCs cultured under conditions that may impact in vitro therapeutic activity, such as aggregate culture, or preconditioning with interferon gamma (IFN- γ)13, is critical for identifying attributes of cell quality. Reducing cell numbers required to perform MSC metabolomic analysis is essential for improving the manufacturing of highly therapeutic MSCs without significantly impeding production. |
| Institute | Georgia Institute of Technology |
| Last Name | Fernandez |
| First Name | Facundo |
| Address | 901 Atlantic Dr NE, Atlanta, GA, 30332, USA |
| fernandez@gatech.edu | |
| Phone | 404-385-4432 |
| Submit Date | 2020-08-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2020-09-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Group |
|---|---|---|
| SA122068 | Blank_3 | Blank |
| SA122069 | Blank_2 | Blank |
| SA122070 | QC_C1 | QC |
| SA122071 | QC_C3 | QC |
| SA122072 | QC_B3 | QC |
| SA122073 | QC_B2 | QC |
| SA122074 | QC_A1 | QC |
| SA122075 | QC_A2 | QC |
| SA122076 | S6_2 | Stimulated |
| SA122077 | S1_1 | Stimulated |
| SA122078 | S6_1 | Stimulated |
| SA122079 | S3_1 | Stimulated |
| SA122080 | S2_3 | Stimulated |
| SA122081 | S2_1 | Stimulated |
| SA122082 | S1_2 | Stimulated |
| SA122083 | S3_2 | Stimulated |
| SA122084 | S4_2 | Stimulated |
| SA122085 | S5_3 | Stimulated |
| SA122086 | S5_1 | Stimulated |
| SA122087 | S4_3 | Stimulated |
| SA122088 | U6_3 | Unstimulated |
| SA122089 | U3_1 | Unstimulated |
| SA122090 | U2_3 | Unstimulated |
| SA122091 | U2_2 | Unstimulated |
| SA122092 | U1_2 | Unstimulated |
| SA122093 | U1_1 | Unstimulated |
| SA122094 | U3_3 | Unstimulated |
| SA122095 | U4_1 | Unstimulated |
| SA122096 | U5_3 | Unstimulated |
| SA122097 | U5_2 | Unstimulated |
| SA122098 | U4_2 | Unstimulated |
| SA122099 | U6_1 | Unstimulated |
| Showing results 1 to 32 of 32 |
