Summary of Study ST001608

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001033. The data can be accessed directly via it's Project DOI: 10.21228/M85M45 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001608
Study TitleComparing gas chromatography with time-of-flight, quadrupole time-of-flight and quadrupole mass spectrometry for stable isotope tracing (part-I)
Study SummaryStable isotope tracers are applied in vivo and in vitro studies to reveal the activity of enzymes and intracellular metabolic pathways. Most often, such tracers are used with gas chromatography coupled to mass spectrometry (GC-MS) due to its ease of operation and reproducible mass spectral databases. Differences in isotope tracer performance of classic GC-quadrupole MS instrument and newer time-of-flight instruments are not well-studied. Here, we used three commercially available instruments for the analysis of identical samples from a stable isotope labeling study that used [U-13C6] d-glucose to investigate the metabolism of Rothia mucilaginosa with respect to 29 amino acids and hydroxyl acids involved in primary metabolism. Overall, all three GC-MS instruments (low-resolution GC-SQ-MS, low-resolution GC-TOF-MS, and high-resolution GC-Q-TOF-MS) can be used to perform stable isotope tracing studies for glycolytic intermediates, TCA metabolites and amino acids, yielding similar biological results, with high-resolution GC-Q-TOF-MS offering additional capabilities to identify chemical structures of unknown compounds that might show significant isotope enrichments in biological studies.
Institute
University of California, Davis
LaboratoryOliver Fiehn
Last NameZhang
First NameYing
AddressWest Coast Metabolomics Center, University of California, Davis, 95616, CA, USA
Emailythzhang@ucdavis.edu
Phone+1-530-754-8258
Submit Date2020-11-19
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2021-05-19
Release Version1
Ying Zhang Ying Zhang
https://dx.doi.org/10.21228/M85M45
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Rothia mucilaginosa (Factor headings shown in green)

mb_sample_id local_sample_id Condition Collection time
SA136482Rothia41_10Ambient 12h
SA136483Rothia42_01Ambient 12h
SA136484Rothia41_09Ambient 12h
SA136485Rothia41_08Ambient 12h
SA136486Rothia41_06Ambient 12h
SA136487Rothia41_07Ambient 12h
SA136488Rothia42_02Ambient 12h
SA136489Rothia42_04Ambient 12h
SA136490Rothia42_08Ambient 12h
SA136491Rothia42_09Ambient 12h
SA136492Rothia42_07Ambient 12h
SA136493Rothia42_06Ambient 12h
SA136494Rothia41_05Ambient 12h
SA136495Rothia42_05Ambient 12h
SA136496Rothia42_03Ambient 12h
SA136497Rothia40_05Ambient 12h
SA136498Rothia40_04Ambient 12h
SA136499Rothia40_06Ambient 12h
SA136500Rothia40_03Ambient 12h
SA136501Rothia40_02Ambient 12h
SA136502Rothia42_10Ambient 12h
SA136503Rothia40_01Ambient 12h
SA136504Rothia40_07Ambient 12h
SA136505Rothia40_08Ambient 12h
SA136506Rothia41_02Ambient 12h
SA136507Rothia41_03Ambient 12h
SA136508Rothia41_01Ambient 12h
SA136509Rothia40_10Ambient 12h
SA136510Rothia40_09Ambient 12h
SA136511Rothia41_04Ambient 12h
SA136512Rothia43_07Ambient 24h
SA136513Rothia45_01Ambient 24h
SA136514Rothia45_02Ambient 24h
SA136515Rothia44_10Ambient 24h
SA136516Rothia44_09Ambient 24h
SA136517Rothia44_07Ambient 24h
SA136518Rothia44_08Ambient 24h
SA136519Rothia45_03Ambient 24h
SA136520Rothia45_04Ambient 24h
SA136521Rothia45_09Ambient 24h
SA136522Rothia45_10Ambient 24h
SA136523Rothia43_01Ambient 24h
SA136524Rothia45_07Ambient 24h
SA136525Rothia45_05Ambient 24h
SA136526Rothia45_06Ambient 24h
SA136527Rothia44_06Ambient 24h
SA136528Rothia45_08Ambient 24h
SA136529Rothia43_05Ambient 24h
SA136530Rothia43_06Ambient 24h
SA136531Rothia43_04Ambient 24h
SA136532Rothia43_02Ambient 24h
SA136533Rothia44_05Ambient 24h
SA136534Rothia43_08Ambient 24h
SA136535Rothia43_03Ambient 24h
SA136536Rothia44_04Ambient 24h
SA136537Rothia44_03Ambient 24h
SA136538Rothia44_02Ambient 24h
SA136539Rothia44_01Ambient 24h
SA136540Rothia43_09Ambient 24h
SA136541Rothia43_10Ambient 24h
SA136542Rothia35_02Ambient 4h
SA136543Rothia35_01Ambient 4h
SA136544Rothia35_05Ambient 4h
SA136545Rothia35_06Ambient 4h
SA136546Rothia35_04Ambient 4h
SA136547Rothia35_03Ambient 4h
SA136548Rothia34_08Ambient 4h
SA136549Rothia35_07Ambient 4h
SA136550Rothia34_05Ambient 4h
SA136551Rothia34_06Ambient 4h
SA136552Rothia34_07Ambient 4h
SA136553Rothia34_09Ambient 4h
SA136554Rothia34_10Ambient 4h
SA136555Rothia36_05Ambient 4h
SA136556Rothia36_08Ambient 4h
SA136557Rothia36_07Ambient 4h
SA136558Rothia36_09Ambient 4h
SA136559Rothia36_10Ambient 4h
SA136560Rothia34_04Ambient 4h
SA136561Rothia36_06Ambient 4h
SA136562Rothia36_04Ambient 4h
SA136563Rothia35_10Ambient 4h
SA136564Rothia36_01Ambient 4h
SA136565Rothia36_02Ambient 4h
SA136566Rothia36_03Ambient 4h
SA136567Rothia35_09Ambient 4h
SA136568Rothia35_08Ambient 4h
SA136569Rothia34_03Ambient 4h
SA136570Rothia34_01Ambient 4h
SA136571Rothia34_02Ambient 4h
SA136572Rothia38_03Ambient 8h
SA136573Rothia38_04Ambient 8h
SA136574Rothia38_06Ambient 8h
SA136575Rothia38_02Ambient 8h
SA136576Rothia38_05Ambient 8h
SA136577Rothia37_10Ambient 8h
SA136578Rothia37_07Ambient 8h
SA136579Rothia37_06Ambient 8h
SA136580Rothia37_08Ambient 8h
SA136581Rothia37_09Ambient 8h
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