Summary of Study ST001613

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001033. The data can be accessed directly via it's Project DOI: 10.21228/M85M45 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001613
Study TitleComparing gas chromatography with time-of-flight, quadrupole time-of-light and quadrupole mass spectrometry for stable isotope tracing (part-II)
Study SummaryStable isotope tracers are applied in vivo and in vitro studies to reveal the activity of enzymes and intracellular metabolic pathways. Most often, such tracers are used with gas chromatography coupled to mass spectrometry (GC-MS) due to its ease of operation and reproducible mass spectral databases. Differences in isotope tracer performance of classic GC-quadrupole MS instrument and newer time-of-flight instruments are not well-studied. Here, we used three commercially available instruments for the analysis of identical samples from a stable isotope labeling study that used [U-13C6] d-glucose to investigate the metabolism of Rothia mucilaginosa with respect to 29 amino acids and hydroxyl acids involved in primary metabolism. Overall, all three GC-MS instruments (low-resolution GC-SQ-MS, low-resolution GC-TOF-MS, and high-resolution GC-Q-TOF-MS) can be used to perform stable isotope tracing studies for glycolytic intermediates, TCA metabolites and amino acids, yielding similar biological results, with high-resolution GC-Q-TOF-MS offering additional capabilities to identify chemical structures of unknown compounds that might show significant isotope enrichments in biological studies.
Institute
University of California, Davis
Last NameZhang
First NameYing
Address451 East Health Science Drive, Davis, CA, 95616, USA
Emailythzhang@ucdavis.edu
Phone1-530-752-8129
Submit Date2020-11-05
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2020-12-10
Release Version1
Ying Zhang Ying Zhang
https://dx.doi.org/10.21228/M85M45
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Bacteria; Subject species: Rothia mucilaginosa (Factor headings shown in green)

mb_sample_id local_sample_id Condition Collection Time
SA136818171015bBG_41_010_1Ambient 12h
SA136819171015bBG_42_010_1Ambient 12h
SA136820171015bBG_41_009_1Ambient 12h
SA136821171015bBG_41_008_1Ambient 12h
SA136822171015bBG_41_006_1Ambient 12h
SA136823171015bBG_41_007_1Ambient 12h
SA136824171015bBG_42_009_1Ambient 12h
SA136825171015bBG_42_003_1Ambient 12h
SA136826171015bBG_41_005_1Ambient 12h
SA136827171015bBG_42_002_1Ambient 12h
SA136828171015bBG_42_004_1Ambient 12h
SA136829171015bBG_42_005_1Ambient 12h
SA136830171015bBG_42_007_1Ambient 12h
SA136831171015bBG_42_006_1Ambient 12h
SA136832171015bBG_42_008_1Ambient 12h
SA136833171015bBG_40_003_1Ambient 12h
SA136834171015bBG_40_007_1Ambient 12h
SA136835171015bBG_40_006_1Ambient 12h
SA136836171015bBG_40_008_1Ambient 12h
SA136837171015bBG_40_009_1Ambient 12h
SA136838171015bBG_42_001_1Ambient 12h
SA136839171015bBG_40_010_1Ambient 12h
SA136840171015bBG_40_005_1Ambient 12h
SA136841171015bBG_40_004_1Ambient 12h
SA136842171015bBG_41_002_1Ambient 12h
SA136843171015bBG_41_003_1Ambient 12h
SA136844171015bBG_41_001_1Ambient 12h
SA136845171015bBG_40_001_1Ambient 12h
SA136846171015bBG_40_002_1Ambient 12h
SA136847171015bBG_41_004_1Ambient 12h
SA136848171015bBG_43_009_1Ambient 24h
SA136849171015bBG_45_001_1Ambient 24h
SA136850171015bBG_45_002_1Ambient 24h
SA136851171015bBG_44_001_1Ambient 24h
SA136852171015bBG_44_002_1Ambient 24h
SA136853171015bBG_44_004_1Ambient 24h
SA136854171015bBG_44_003_1Ambient 24h
SA136855171015bBG_45_003_1Ambient 24h
SA136856171015bBG_45_004_1Ambient 24h
SA136857171015bBG_43_010_1Ambient 24h
SA136858171015bBG_45_010_1Ambient 24h
SA136859171015bBG_45_008_1Ambient 24h
SA136860171015bBG_45_007_1Ambient 24h
SA136861171015bBG_45_005_1Ambient 24h
SA136862171015bBG_45_006_1Ambient 24h
SA136863171015bBG_44_005_1Ambient 24h
SA136864171015bBG_45_009_1Ambient 24h
SA136865171015bBG_44_006_1Ambient 24h
SA136866171015bBG_43_004_1Ambient 24h
SA136867171015bBG_43_006_1Ambient 24h
SA136868171015bBG_43_007_1Ambient 24h
SA136869171015bBG_43_008_1Ambient 24h
SA136870171015bBG_43_003_1Ambient 24h
SA136871171015bBG_43_005_1Ambient 24h
SA136872171015bBG_44_008_1Ambient 24h
SA136873171015bBG_43_002_1Ambient 24h
SA136874171015bBG_44_009_1Ambient 24h
SA136875171015bBG_44_007_1Ambient 24h
SA136876171015bBG_44_010_1Ambient 24h
SA136877171015bBG_43_001_1Ambient 24h
SA136878171012bBG_34_010_1Ambient 4h
SA136879171012bBG_34_009_1Ambient 4h
SA136880171012bBG_35_001_1Ambient 4h
SA136881171012bBG_35_003_1Ambient 4h
SA136882171012bBG_35_004_1Ambient 4h
SA136883171012bBG_35_002_1Ambient 4h
SA136884171012bBG_34_002_1Ambient 4h
SA136885171012bBG_34_003_1Ambient 4h
SA136886171012bBG_35_006_1Ambient 4h
SA136887171012bBG_34_004_1Ambient 4h
SA136888171012bBG_34_005_1Ambient 4h
SA136889171012bBG_34_007_1Ambient 4h
SA136890171012bBG_34_006_1Ambient 4h
SA136891171012bBG_34_008_1Ambient 4h
SA136892171012bBG_36_007_1Ambient 4h
SA136893171012bBG_36_004_1Ambient 4h
SA136894171012bBG_36_005_1Ambient 4h
SA136895171012bBG_36_003_1Ambient 4h
SA136896171012bBG_36_002_1Ambient 4h
SA136897171012bBG_34_001_1Ambient 4h
SA136898171012bBG_36_001_1Ambient 4h
SA136899171012bBG_36_006_1Ambient 4h
SA136900171012bBG_36_008_1Ambient 4h
SA136901171012bBG_35_009_1Ambient 4h
SA136902171012bBG_35_008_1Ambient 4h
SA136903171012bBG_35_010_1Ambient 4h
SA136904171012bBG_36_010_1Ambient 4h
SA136905171012bBG_36_009_1Ambient 4h
SA136906171012bBG_35_007_1Ambient 4h
SA136907171012bBG_35_005_1Ambient 4h
SA136908171012bBG_37_010_1Ambient 8h
SA136909171012bBG_37_009_1Ambient 8h
SA136910171012bBG_38_001_1Ambient 8h
SA136911171012bBG_38_002_1Ambient 8h
SA136912171012bBG_38_004_1Ambient 8h
SA136913171012bBG_38_003_1Ambient 8h
SA136914171012bBG_37_008_1Ambient 8h
SA136915171012bBG_37_007_1Ambient 8h
SA136916171012bBG_37_002_1Ambient 8h
SA136917171012bBG_39_010_1Ambient 8h
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