Summary of Study ST001622

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001041. The data can be accessed directly via it's Project DOI: 10.21228/M84M6M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001622
Study TitleIsotope Tracing Analysis in Intestinal Organoids with fructose
Study SummaryWe isolated and cultured Intestinal Organoids from Mouse. To trace the metabolism of fructose, intestinal organoids were cultured in unlabeled fructose-containing DMEM/F12 medium (10 mM D-fructose, 0 mM D-glucose, 2.5 mM L-glutamine) for 12 hr, and then switched into 13C labeled fructose-containing medium (10 mM U-[13C] D-fructose, Sigma-aldrich) for 6 hr.
Institute
China Pharmaceutical University
DepartmentSchool of Medicine
LaboratoryMetabonomics
Last NameHou
First NameYuanlong
Addresstongjiaxiang, nanjing, jiangsu, 210000, China
Emailjian2103@163.com
Phone18851105337
Submit Date2020-12-09
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2020-12-16
Release Version1
Yuanlong Hou Yuanlong Hou
https://dx.doi.org/10.21228/M84M6M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA13792113C Fru113C Fructose
SA13792213C Fru313C Fructose
SA13792313C Fru213C Fructose
SA137924Fru3Fructose
SA137925Fru1Fructose
SA137926Fru2Fructose
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