Summary of Study ST001687
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001085. The data can be accessed directly via it's Project DOI: 10.21228/M8FT4Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001687 |
| Study Title | Non-transformed cells respond to fat by inducing glucose metabolism |
| Study Summary | C57BL/6N mice were obtained from the KU Leuven animal laboratory. 2-week old mice were injected i.p. diethylnitrosamine (DEN, 25 mg/Kg) in phosphate buffered saline (3.17 mg/ml).s At 6-weeks of age, mice were randomized into two groups: control diet (CD, E15742-33 ssniff Spezialdiäten GmbH) or high fat diet (HFD, S8655-E220 sniff Spezialdiäten GmbH). At endpoint, mice were sacrificed by injecting approximately 50 µl of a 60 mg/ml Nembutal solution (Vetoquinol). Tissues were immediately excised, washed in ice-cold saline, placed into pre-labelled bags and frozen using a liquid nitrogen-cooled biosqueezer. The bags were then placed in liquid nitrogen until all collections were finished and finally stored at -80°C until further processing. Where necessary tumors were rapidly separated from normal tissue prior to freezing with tumor and normal tissue being stored separately. |
| Institute | VIB-KU Leuven Center for Cancer Biology |
| Department | Department of Oncology |
| Laboratory | Laboratory of Cellular Metabolism and Metabolic Regulation |
| Last Name | Fendt |
| First Name | Sarah-Maria |
| Address | ON IV Herestraat 49 - box 912 3000 Leuven |
| sarah-maria.fendt@kuleuven.be | |
| Phone | +32 16 37 32 61 |
| Submit Date | 2021-02-04 |
| Num Groups | 4 |
| Total Subjects | 16 |
| Num Males | 16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-02-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Tissue | Diet | DEN |
|---|---|---|---|---|
| SA155491 | CD-PBS_3.1.2 | Liver | CD | No |
| SA155492 | CD-PBS_3.2.3 | Liver | CD | No |
| SA155493 | CD-PBS_3.2.1 | Liver | CD | No |
| SA155494 | CD-PBS_3.1.4 | Liver | CD | No |
| SA155495 | HFD-PBS_3.5.3 | Liver | HFD | No |
| SA155496 | HFD-PBS_3.4.4 | Liver | HFD | No |
| SA155497 | HFD-PBS_3.4.3 | Liver | HFD | No |
| SA155498 | HFD-PBS_3.5.1 | Liver | HFD | No |
| SA155499 | HFD-DEN_3D20.2L | Liver | HFD | Yes |
| SA155500 | HFD-DEN_3D20.1L | Liver | HFD | Yes |
| SA155501 | HFD-DEN_3D19.1L | Liver | HFD | Yes |
| SA155502 | HFD-DEN_3D19.2L | Liver | HFD | Yes |
| SA155503 | HFD-DEN_3D19.1T | Tumor | HFD | Yes |
| SA155504 | HFD-DEN_3D20.2T | Tumor | HFD | Yes |
| SA155505 | HFD-DEN_3D19.2T | Tumor | HFD | Yes |
| SA155506 | HFD-DEN_3D20.1T | Tumor | HFD | Yes |
| Showing results 1 to 16 of 16 |
