Summary of Study ST001765

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001765
Study TitleOptimization of redox metabolite detection in mammalian cells (part I)
Study SummaryConditions were tested to optimize number of cells and extraction buffer for the detection of redox reactive metabolites from mammalian cells. Four different extraction buffers were compared. Derivatization of glutathione was explored as a condition as well. This is an independent repeat.
Institute
Boston Children's Hospital, Harvard Medical School
DepartmentPathology
LaboratoryNaama Kanarek
Last NamePetrova
First NameBoryana
Address300 Longwood Av, Boston, MA, 2115, USA
Emailboryana.petrova@childrens.harvard.edu
Phone6173557433
Submit Date2021-04-21
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-05-17
Release Version1
Boryana Petrova Boryana Petrova
https://dx.doi.org/10.21228/M81D6M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA164321pooled sample QC f-
SA164322pooled sample QC e-
SA164323pooled sample QC 1/3 dilution-
SA164324pooled sample QC 1/10 dilution-
SA164325blank 2-
SA164326pooled sample QC 1/10 dilution a-
SA164327pooled sample QC d-
SA164328pooled sample QC a-
SA164329blank 3-
SA164330blank 1-
SA164331blank 4-
SA164332pooled sample QC 3x loaded-
SA164333pooled sample QC b-
SA164334pooled sample QC c-
SA164300442_1M_1"buffer A, 1Milion cells"
SA164301442_1M_1.1"buffer A, 1Milion cells"
SA164302442_2M_1"buffer A, 2Milion cells"
SA164303buffer B_1M_2"buffer B, 1Milion cells"
SA164304buffer B_1M_1"buffer B, 1Milion cells"
SA164305buffer B_2M_1"buffer B, 2Milion cells"
SA164306buffer B_2M_2"buffer B, 2Milion cells"
SA164307buffer B_5M_2"buffer B, 5Milion cells"
SA164308buffer B_5M_1"buffer B, 5Milion cells"
SA164309buffer C_1M_2"buffer C, 1Milion cells"
SA164310buffer C_1M_1"buffer C, 1Milion cells"
SA164311buffer C_2M_1"buffer C, 2Milion cells"
SA164312buffer C_2M_2"buffer C, 2Milion cells"
SA164313buffer C_5M_2"buffer C, 5Milion cells"
SA164314buffer C_5M_1"buffer C, 5Milion cells"
SA164315buffer C_Ell_1M_1"buffer C, plus Ellmans, 1Milion cells"
SA164316buffer C_Ell_1M_2"buffer C, plus Ellmans, 1Milion cells"
SA164317buffer C_Ell_2M_1"buffer C, plus Ellmans, 2Milion cells"
SA164318buffer C_Ell_2M_2"buffer C, plus Ellmans, 2Milion cells"
SA164319buffer C_Ell_5M_1"buffer C, plus Ellmans, 5Milion cells"
SA164320buffer C_Ell_5M_2"buffer C, plus Ellmans, 5Milion cells"
Showing results 1 to 35 of 35
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