Summary of Study ST001835

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001159. The data can be accessed directly via it's Project DOI: 10.21228/M8WH6D This work is supported by NIH grant, U2C- DK119886.

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Study IDST001835
Study TitleUse of Integrated Metabolomics, Transcriptomics, and Signal Protein Profile to Characterize the Effector Function and Associated Metabotype of Polarized Macrophage Phenotypes
Study TypeEx vivo macrophage polarization metabotyping
Study SummaryMacrophages (MΦs) display remarkable plasticity and the ability to activate diverse responses to a host of intracellular and external stimuli. Despite extensive characterization of M1 MΦs and a broad set of M2 MΦs, comprehensive characterization of functional phenotype and associated metabotype driving this diverse MΦ activation remains. Herein, we utilized an ex vivo model to produce six MΦ functional phenotypes. Isolated CD14+ PBMCs were differentiated into resting M0 MΦs, and then polarized into M1 (IFN-γ/LPS), M2a (IL-4/IL-13), M2b (IC/LPS), M2c (IL-10), and M2d (IL-6/LIF) MΦs. The MΦs were profiled using a bioanalyte matrix of four cell surface markers, ~50 secreted proteins, ~800 expressed myeloid genes, and ~450 identified metabolites relative to M0 MΦs. Signal protein and expressed gene profiles grouped the MΦs into inflammatory (M1 and M2b) and wound resolution (M2a, M2c, and M2d) phenotypes; however, each had a unique metabolic profile. While both M1 and M2b MΦs shared metabotype profiles consistent with an inflammatory signature; key differences were observed in the TCA cycle, FAO, and OXPHOS. Additionally, M2a, M2c, and M2d MΦs all profiled as tissue repair MΦs; however, metabotype differences were observed in multiple pathways including hexosamine, polyamine, and fatty acid metabolism. These metabolic and other key functional distinctions suggest phagocytic and proliferative functions for M2a MΦs, and angiogenesis and ECM assembly capabilities for M2b, M2c, and M2d MΦs. By integrating metabolomics into a systems analysis of MΦ phenotypes, we provide the most comprehensive map of MΦ diversity to date, along with the global metabolic shifts that correlate to MΦ functional plasticity in these phenotypes.
Institute
Idaho Veterans Research and Education Foundation
DepartmentResearch
LaboratoryAmmons
Last NameAmmons
First NameMary Cloud
AddressMail Stop 151, Bldg 117, 500 W. Fort St. Boise, Idaho 83702
EmailMaryCloud.AmmonsAnderson@va.gov
Phone208-422-1219
Submit Date2021-06-18
Analysis Type DetailLC-MS
Release Date2021-09-17
Release Version1
Mary Cloud Ammons Mary Cloud Ammons
https://dx.doi.org/10.21228/M8WH6D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id hours treatment
SA170650M2c_037_08141824 IL10
SA170651M2c_016_09241824 IL10
SA170652M2c_038_08141824 IL10
SA170653M2a_016_09241824 IL4_IL13
SA170654M2a_037_08141824 IL4_IL13
SA170655M2a_038_08141824 IL4_IL13
SA170656M2d_037_08141824 IL6_LIF
SA170657M2d_016_09241824 IL6_LIF
SA170658M2d_038_08141824 IL6_LIF
SA170659M2b_038_08141824 LPS_IC
SA170660M2b_016_09241824 LPS_IC
SA170661M2b_037_08141824 LPS_IC
SA170662M1_016_09241824 LPS_IFNG
SA170663M1_037_08141824 LPS_IFNG
SA170664M1_038_08141824 LPS_IFNG
SA170665M0_016_09241824 NT
SA170666M0_038_08141824 NT
SA170667M0_037_08141824 NT
SA170668M2c_016_07121872 IL10
SA170669M2c_017_06281872 IL10
SA170670M2c_028_07051872 IL10
SA170671M2a_028_07051872 IL4_IL13
SA170672M2a_016_07121872 IL4_IL13
SA170673M2a_017_06281872 IL4_IL13
SA170674M2d_017_06281872 IL6_LIF
SA170675M2d_028_07051872 IL6_LIF
SA170676M2d_016_07121872 IL6_LIF
SA170677M2b_028_07051872 LPS_IC
SA170678M2b_016_07121872 LPS_IC
SA170679M2b_017_06281872 LPS_IC
SA170680M1_017_06281872 LPS_IFNG
SA170681M1_016_07121872 LPS_IFNG
SA170682M1_028_07051872 LPS_IFNG
SA170683M0_016_07121872 NT
SA170684M0_017_06281872 NT
SA170685M0_028_07051872 NT
Showing results 1 to 36 of 36
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