Summary of Study ST002038

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001288. The data can be accessed directly via it's Project DOI: 10.21228/M8771V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002038
Study TitleIrradiation causes alterations of polyamine, purine and sulfur metabolism in red blood cells and multiple organs (Duodenum)
Study SummaryInvestigating the metabolic effects of radiation is critical to understand the impact of radiotherapy (e.g., for bone marrow irradiation prior to hematopoietic stem cell transplantation in the clinic or in laboratory studies), space travel, and exposure to environmental radiation. In patients undergoing hemopoietic stem cell transplantation, iron overload is a common risk factor for poor outcomes. Previous studies assert that both irradiation and iron independently modulate tryptophan and indole metabolism of the microbiome, which may in turn impact host immune response. However, no studies have interrogated the multi-organ effects of these treatments concurrently. Herein, we use a model that recapitulate transfusional iron overload, a condition often observed in chronically transfused patients with thalassemia, sickle cell disease, or myelodysplastic syndrome. We applied an omics approach to investigate the impact of both iron load and irradiation on the host metabolome. Our results revealed dose-dependent effects of irradiation in red blood cells (RBC), plasma, spleen, and liver energy and redox metabolism. Increases in polyamines and purine salvage metabolites were observed in organs with high oxygen consumption including the heart, kidney, and brain. Irradiation also impacted the metabolism of the duodenum, colon, and stool, suggesting a potential effect on the microbiome. Iron infusion affected the respose to radiation in the organs and blood, especially in RBC polyamine metabolism and spleen antioxidant metabolism, and affected glucose, sulfur (especially methionine and glutathione systems) and tryptophan metabolism in the liver, stool, and brain. Together, the results suggest that radiation impacts metabolism on a multi-organ level with a significant interaction of host iron status.
Institute
University of Colorado Anschutz Medical Campus
Last NameRoy
First NameMicaela
Address13001 E 17th Pl, Aurora, CO 80045
Emailmicaela.roy@cuanschutz.edu
Phone303-724-3339
Submit Date2021-12-28
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-01-21
Release Version1
Micaela Roy Micaela Roy
https://dx.doi.org/10.21228/M8771V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Radiation Dose
SA19166149IV iron Gy10
SA19166250IV iron Gy10
SA19166347IV iron Gy10
SA19166448IV iron Gy10
SA19166546IV iron Gy10
SA19166657IV iron Gy11
SA19166758IV iron Gy11
SA19166856IV iron Gy11
SA19166959IV iron Gy11
SA19167060IV iron Gy11
SA19167119IV iron Gy7
SA19167220IV iron Gy7
SA19167318IV iron Gy7
SA19167417IV iron Gy7
SA19167516IV iron Gy7
SA19167628IV iron Gy8
SA19167729IV iron Gy8
SA19167830IV iron Gy8
SA19167926IV iron Gy8
SA19168027IV iron Gy8
SA19168137IV iron Gy9
SA19168239IV iron Gy9
SA19168340IV iron Gy9
SA19168436IV iron Gy9
SA19168538IV iron Gy9
SA1916866IV iron no irradiated
SA1916877IV iron no irradiated
SA1916888IV iron no irradiated
SA19168910IV iron no irradiated
SA1916909IV iron no irradiated
SA19169141Saline Gy10
SA19169244Saline Gy10
SA19169345Saline Gy10
SA19169443Saline Gy10
SA19169542Saline Gy10
SA19169651Saline Gy11
SA19169753Saline Gy11
SA19169854Saline Gy11
SA19169955Saline Gy11
SA19170052Saline Gy11
SA19170115Saline Gy7
SA19170214Saline Gy7
SA19170313Saline Gy7
SA19170411Saline Gy7
SA19170525Saline Gy8
SA19170622Saline Gy8
SA19170724Saline Gy8
SA19170823Saline Gy8
SA19170921Saline Gy8
SA19171032Saline Gy9
SA19171131Saline Gy9
SA19171235Saline Gy9
SA19171333Saline Gy9
SA19171434Saline Gy9
SA1917152Saline no irradiated
SA1917163Saline no irradiated
SA1917171Saline no irradiated
SA1917184Saline no irradiated
Showing results 1 to 58 of 58
  logo