Summary of Study ST002040

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001288. The data can be accessed directly via it's Project DOI: 10.21228/M8771V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002040
Study TitleIrradiation causes alterations of polyamine, purine and sulfur metabolism in red blood cells and multiple organs (Colon)
Study SummaryInvestigating the metabolic effects of radiation is critical to understand the impact of radiotherapy (e.g., for bone marrow irradiation prior to hematopoietic stem cell transplantation in the clinic or in laboratory studies), space travel, and exposure to environmental radiation. In patients undergoing hemopoietic stem cell transplantation, iron overload is a common risk factor for poor outcomes. Previous studies assert that both irradiation and iron independently modulate tryptophan and indole metabolism of the microbiome, which may in turn impact host immune response. However, no studies have interrogated the multi-organ effects of these treatments concurrently. Herein, we use a model that recapitulate transfusional iron overload, a condition often observed in chronically transfused patients with thalassemia, sickle cell disease, or myelodysplastic syndrome. We applied an omics approach to investigate the impact of both iron load and irradiation on the host metabolome. Our results revealed dose-dependent effects of irradiation in red blood cells (RBC), plasma, spleen, and liver energy and redox metabolism. Increases in polyamines and purine salvage metabolites were observed in organs with high oxygen consumption including the heart, kidney, and brain. Irradiation also impacted the metabolism of the duodenum, colon, and stool, suggesting a potential effect on the microbiome. Iron infusion affected the respose to radiation in the organs and blood, especially in RBC polyamine metabolism and spleen antioxidant metabolism, and affected glucose, sulfur (especially methionine and glutathione systems) and tryptophan metabolism in the liver, stool, and brain. Together, the results suggest that radiation impacts metabolism on a multi-organ level with a significant interaction of host iron status.
Institute
University of Colorado Anschutz Medical Campus
Last NameRoy
First NameMicaela
Address1635 Aurora Ct, Aurora, CO, 80045, USA
Emailmicaela.roy@cuanschutz.edu
Phone303-724-3339
Submit Date2021-12-28
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-01-21
Release Version1
Micaela Roy Micaela Roy
https://dx.doi.org/10.21228/M8771V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Condition Radiation Dose
SA19177749IV iron Gy10
SA19177850IV iron Gy10
SA19177947IV iron Gy10
SA19178048IV iron Gy10
SA19178146IV iron Gy10
SA19178257IV iron Gy11
SA19178358IV iron Gy11
SA19178456IV iron Gy11
SA19178559IV iron Gy11
SA19178660IV iron Gy11
SA19178719IV iron Gy7
SA19178820IV iron Gy7
SA19178918IV iron Gy7
SA19179016IV iron Gy7
SA19179117IV iron Gy7
SA19179228IV iron Gy8
SA19179326IV iron Gy8
SA19179429IV iron Gy8
SA19179527IV iron Gy8
SA19179637IV iron Gy9
SA19179739IV iron Gy9
SA19179840IV iron Gy9
SA19179936IV iron Gy9
SA19180038IV iron Gy9
SA1918016IV iron no irradiated
SA1918027IV iron no irradiated
SA1918038IV iron no irradiated
SA19180410IV iron no irradiated
SA1918059IV iron no irradiated
SA19180641Saline Gy10
SA19180744Saline Gy10
SA19180845Saline Gy10
SA19180943Saline Gy10
SA19181042Saline Gy10
SA19181151Saline Gy11
SA19181252Saline Gy11
SA19181354Saline Gy11
SA19181455Saline Gy11
SA19181553Saline Gy11
SA19181615Saline Gy7
SA19181714Saline Gy7
SA19181813Saline Gy7
SA19181911Saline Gy7
SA19182024Saline Gy8
SA19182122Saline Gy8
SA19182223Saline Gy8
SA19182325Saline Gy8
SA19182421Saline Gy8
SA19182532Saline Gy9
SA19182631Saline Gy9
SA19182735Saline Gy9
SA19182833Saline Gy9
SA19182934Saline Gy9
SA1918302Saline no irradiated
SA1918313Saline no irradiated
SA1918321Saline no irradiated
SA1918334Saline no irradiated
Showing results 1 to 57 of 57
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