Summary of Study ST002111
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001337. The data can be accessed directly via it's Project DOI: 10.21228/M8WM52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002111 |
| Study Title | Metabolomics dataset of optogenetic axon regenerative mouse model post optic nerve crush |
| Study Summary | This metabolite dataset was collected from bacterial channel rhodopsin expressing transgenic mouse models subjected to optic nerve crush (ONC) with subsequent stimulation with light (promoting regeneration) or non-stimulation (lacking axon regeneration). ONC retains retinal ganglion cells within the retina, while degenerating axons. In transgenic bacterial channel rhodopsin expressing cells, light stimulation promotes regeneration. Genetically matched wild-type uninjured optic nerves, or control transgenic mice, were also analyzed. Metabolites were carefully extracted from finely minced optic nerve tissue using a solvent system (initial separation using 1:1 Methanol and H2O and second extraction using 8:1:1 of Acetonitrile:Acetone:Methanol). Untargeted liquid chromatography-mass spectrometry (LC-MS/MS) profiling was performed using fractionation on a Vanquish Horizon Binary UHPLC. Subsequent analyses were performed on an inline coupled Q-Exactive Orbitrap instrument. Metabolites were identified using Compound DiscovererTM software. Statistical analysis was performed using MetaboAnalyst 5.0. |
| Institute | University of Miami |
| Department | Ophthalmology |
| Last Name | Bhattacharya |
| First Name | Sanjoy K. |
| Address | 1638 NW 10th Avenue, Room 706-A, Miami, FL 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | (305) 482-4103 |
| Submit Date | 2022-03-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-04-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA202442 | ChR_1WPC_NS_NEG_(2) | ChR 1WPC NS |
| SA202443 | ChR_1WPC_NS_NEG_(3) | ChR 1WPC NS |
| SA202444 | ChR_1WPC_NS_POS_(1) | ChR 1WPC NS |
| SA202445 | ChR_1WPC_NS_NEG_(1) | ChR 1WPC NS |
| SA202446 | ChR_1WPC_NS_POS_(2) | ChR 1WPC NS |
| SA202447 | ChR_1WPC_NS_POS_(3) | ChR 1WPC NS |
| SA202448 | ChR_1WPC_PS_POS_(1) | ChR 1WPC PS |
| SA202449 | ChR_1WPC_PS_POS_(3) | ChR 1WPC PS |
| SA202450 | ChR_1WPC_PS_NEG_(2) | ChR 1WPC PS |
| SA202451 | ChR_1WPC_PS_NEG_(3) | ChR 1WPC PS |
| SA202452 | ChR_1WPC_PS_POS_(2) | ChR 1WPC PS |
| SA202453 | ChR_1WPC_PS_NEG_(1) | ChR 1WPC PS |
| SA202454 | ChR_2WPC_NS_POS_(3) | ChR 2WPC NS |
| SA202455 | ChR_2WPC_NS_NEG_(2) | ChR 2WPC NS |
| SA202456 | ChR_2WPC_NS_NEG_(3) | ChR 2WPC NS |
| SA202457 | ChR_2WPC_NS_POS_(1) | ChR 2WPC NS |
| SA202458 | ChR_2WPC_NS_POS_(2) | ChR 2WPC NS |
| SA202459 | ChR_2WPC_NS_NEG_(1) | ChR 2WPC NS |
| SA202460 | ChR_2WPC_PS_POS_(2) | ChR 2WPC PS |
| SA202461 | ChR_2WPC_PS_POS_(1) | ChR 2WPC PS |
| SA202462 | ChR_2WPC_PS_NEG_(2) | ChR 2WPC PS |
| SA202463 | ChR_2WPC_PS_NEG_(3) | ChR 2WPC PS |
| SA202464 | ChR_2WPC_PS_POS_(3) | ChR 2WPC PS |
| SA202465 | ChR_2WPC_PS_NEG_(1) | ChR 2WPC PS |
| SA202466 | ChR_NC_NS_POS_(8) | ChR NC NS |
| SA202467 | ChR_NC_NS_NEG_(2) | ChR NC NS |
| SA202468 | ChR_NC_NS_POS_(4) | ChR NC NS |
| SA202469 | ChR_NC_NS_POS_(5) | ChR NC NS |
| SA202470 | ChR_NC_NS_POS_(2) | ChR NC NS |
| SA202471 | ChR_NC_NS_NEG_(3) | ChR NC NS |
| SA202472 | ChR_NC_NS_NEG_(4) | ChR NC NS |
| SA202473 | ChR_NC_NS_NEG_(8) | ChR NC NS |
| SA202474 | ChR_NC_NS_POS_(3) | ChR NC NS |
| SA202475 | ChR_NC_NS_NEG_(7) | ChR NC NS |
| SA202476 | ChR_NC_NS_NEG_(6) | ChR NC NS |
| SA202477 | ChR_NC_NS_NEG_(5) | ChR NC NS |
| SA202478 | ChR_NC_NS_POS_(6) | ChR NC NS |
| SA202479 | ChR_NC_NS_NEG_(1) | ChR NC NS |
| SA202480 | ChR_NC_NS_POS_(7) | ChR NC NS |
| SA202481 | ChR_NC_NS_POS_(1) | ChR NC NS |
| SA202482 | WT_1WPC_NS_POS_(4) | WT 1WPC NS |
| SA202483 | WT_1WPC_NS_POS_(3) | WT 1WPC NS |
| SA202484 | WT_1WPC_NS_NEG_(4) | WT 1WPC NS |
| SA202485 | WT_1WPC_NS_NEG_(3) | WT 1WPC NS |
| SA202486 | WT_1WPC_NS_NEG_(1) | WT 1WPC NS |
| SA202487 | WT_1WPC_NS_NEG_(2) | WT 1WPC NS |
| SA202488 | WT_1WPC_NS_POS_(2) | WT 1WPC NS |
| SA202489 | WT_1WPC_NS_POS_(1) | WT 1WPC NS |
| SA202490 | WT_2WPC_NS_NEG_(1) | WT 2WPC NS |
| SA202491 | WT_2WPC_NS_POS_(3) | WT 2WPC NS |
| SA202492 | WT_2WPC_NS_POS_(1) | WT 2WPC NS |
| SA202493 | WT_2WPC_NS_POS_(2) | WT 2WPC NS |
| SA202494 | WT_2WPC_NS_NEG_(3) | WT 2WPC NS |
| SA202495 | WT_2WPC_NS_NEG_(2) | WT 2WPC NS |
| SA202496 | WT_NC_NS_NEG_(5) | WT NC NS |
| SA202497 | WT_NC_NS_NEG_(6) | WT NC NS |
| SA202498 | WT_NC_NS_NEG_(2) | WT NC NS |
| SA202499 | WT_NC_NS_NEG_(4) | WT NC NS |
| SA202500 | WT_NC_NS_NEG_(3) | WT NC NS |
| SA202501 | WT_NC_NS_POS_(1) | WT NC NS |
| SA202502 | WT_NC_NS_POS_(5) | WT NC NS |
| SA202503 | WT_NC_NS_POS_(6) | WT NC NS |
| SA202504 | WT_NC_NS_POS_(4) | WT NC NS |
| SA202505 | WT_NC_NS_POS_(3) | WT NC NS |
| SA202506 | WT_NC_NS_POS_(2) | WT NC NS |
| SA202507 | WT_NC_NS_NEG_(1) | WT NC NS |
| Showing results 1 to 66 of 66 |
