Summary of Study ST002125
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001348. The data can be accessed directly via it's Project DOI: 10.21228/M8GD8D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002125 |
| Study Title | Amino acids and TCA substrates in hematopoietic cells (Part2) |
| Study Summary | This study uses [13C,15N] labeled amino acids to study the amino acid consumption and their catabolism into tricarboxylic acid cycle substrates in hematopoietic stem cells, hemopoietic progenitors, and differentiated hematopoietic cells under different conditions, such as homeostasis and proliferation and with drug treatment. |
| Institute | Sun Yat-sen University |
| Last Name | Zhao |
| First Name | Meng |
| Address | Zhongshan 2nd Road |
| zhaom38@mail.sysu.edu.cn | |
| Phone | 18138799889 |
| Submit Date | 2022-04-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2022-11-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Source_Name[gating] | Treatment | Batch | Note |
|---|---|---|---|---|---|
| SA204320 | BM_supernatant_5FU_1 | Bone marrow supernatant | 5 FU 2 weeks | 1c | Unlabeled AAs were used for total AA (FigS2.D) |
| SA204321 | BM_supernatant_5FU_2 | Bone marrow supernatant | 5 FU 2 weeks | 1c | Unlabeled AAs were used for total AA (FigS2.D) |
| SA204322 | BM_supernatant_5FU_3 | Bone marrow supernatant | 5 FU 2 weeks | 1c | Unlabeled AAs were used for total AA (FigS2.D) |
| SA204323 | BM_supernatant_Ctrl_1 | Bone marrow supernatant | HSC Basal | 1c | Unlabeled AAs were used for total AA (FigS2.D) |
| SA204324 | BM_supernatant_Ctrl_2 | Bone marrow supernatant | HSC Basal | 1c | Unlabeled AAs were used for total AA (FigS2.D) |
| SA204325 | BM_supernatant_Ctrl_3 | Bone marrow supernatant | HSC Basal | 1c | Unlabeled AAs were used for total AA (FigS2.D) |
| SA204326 | HSC_5FU_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| SA204327 | HSC_5FU_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| SA204328 | HSC_5FU_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| SA204329 | HSC_5FU_0h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204330 | HSC_5FU_0h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204331 | HSC_5FU_0h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204332 | HSC_5FU_12h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204333 | HSC_5FU_12h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204334 | HSC_5FU_12h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204335 | HSC_5FU_6h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204336 | HSC_5FU_6h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204337 | HSC_5FU_6h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | 5 FU 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204338 | HSC_Cultured_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C) and TCA substrate levels (Fig2.K). |
| SA204339 | HSC_Cultured_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C) and TCA substrate levels (Fig2.K). |
| SA204340 | HSC_Cultured_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C) and TCA substrate levels (Fig2.K). |
| SA204341 | HSC_Cultured_6h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204342 | HSC_Cultured_0h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204343 | HSC_Cultured_0h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204344 | HSC_Cultured_0h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204345 | HSC_Cultured_12h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204346 | HSC_Cultured_12h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204347 | HSC_Cultured_12h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204348 | HSC_Cultured_6h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204349 | HSC_Cultured_6h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Ex vivo culture 2 weeks, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig2.D). |
| SA204353 | HSC_Ctrl_0h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204354 | HSC_Ctrl_0h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204355 | HSC_Ctrl_0h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204356 | HSC_Ctrl_12h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204357 | HSC_Ctrl_12h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204358 | HSC_Ctrl_12h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204359 | HSC_Ctrl_6h_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204360 | HSC_Ctrl_6h_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204361 | HSC_Ctrl_6h_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | 1b | [13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig1.C, Fig2.D). |
| SA204350 | HSC_Ctrl_3 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| SA204351 | HSC_Ctrl_1 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| SA204352 | HSC_Ctrl_2 | Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | HSC Basal or Ctrl, [13C, 15N] AAs were incorporated for 1 h | 1c | [13C, 15N] AAs were used for AA uptake (Fig2.B, Fig5M); unlabeled AAs and TCA substrate were used for total AA levels (Fig2.C, Fig5K) and TCA substrate levels (Fig2.K, Fig5J). |
| Showing results 1 to 42 of 42 |
