Summary of Study ST002152

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001365. The data can be accessed directly via it's Project DOI: 10.21228/M88Q5H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002152
Study TitleMetabolomics analysis of mouse liver with or without SIRT5 deficiency
Study Summary8 Wild-type (WT) and 8 Sirt5-/- (SIRT5 KO) mice on 129 background were maintained on a standard chow diet (Teklad Global 18% Protein Rodent Diet, ENVIGO, Cat.#2018) until they were put on euthanized. Liver metabolites were extracted (5-10 mg) were extracted using 80% methanol/water as the extraction solvent. Metabolite extract was split into two tubes (one for polar metabolite analysis and the other one for acyl-CoA analysis), and then extraction solvent was evaporated using a speed vacuum concentrator. Dry pellets were stored in −80 °C freezer until ready for LC-MS analysis. For acyl-CoA analysis, dry pellets were reconstituted into 30 μL of sample solvent (water containing 50 mM ammonium acetate) per 6 mg tissue, and 8 μL was injected into the LC-MS. For non-acyl-CoA polar metabolite analysis, pellets were reconstituted into 30 μL of sample solvent (water:methanol:acetonitrile, 2:1:1, v/v/v) per 3 mg tissue, and 3 μL was injected into the LC-MS.
Institute
North Carolina State University
Last NameLiu
First NameXiaojing
AddressPolk Hall, RM 128
Emailxliu68@ncsu.edu
Phone9195154387
Submit Date2022-04-14
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-05-09
Release Version1
Xiaojing Liu Xiaojing Liu
https://dx.doi.org/10.21228/M88Q5H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample Source
SA206325KO_6.rawSIRT5 knockout
SA206326KO_5.rawSIRT5 knockout
SA206327KO_4.rawSIRT5 knockout
SA206328KO_7.rawSIRT5 knockout
SA206329KO_8.rawSIRT5 knockout
SA206330KO_1_luna.rawSIRT5 knockout
SA206331KO_2_luna.rawSIRT5 knockout
SA206332KO_3.rawSIRT5 knockout
SA206333KO_2.rawSIRT5 knockout
SA206334KO_7_luna.rawSIRT5 knockout
SA206335KO_8_luna.rawSIRT5 knockout
SA206336KO_6_luna.rawSIRT5 knockout
SA206337KO_5_luna.rawSIRT5 knockout
SA206338KO_1.rawSIRT5 knockout
SA206339KO_3_luna.rawSIRT5 knockout
SA206340KO_4_luna.rawSIRT5 knockout
SA206341WT_7_luna.rawWild type
SA206342WT_8_luna.rawWild type
SA206343WT_6_luna.rawWild type
SA206344WT_1_luna.rawWild type
SA206345WT_5.rawWild type
SA206346WT_6.rawWild type
SA206347WT_4.rawWild type
SA206348WT_3.rawWild type
SA206349WT_2.rawWild type
SA206350WT_7.rawWild type
SA206351WT_8.rawWild type
SA206352WT_4_luna.rawWild type
SA206353WT_3_luna.rawWild type
SA206354WT_2_luna.rawWild type
SA206355WT_1.rawWild type
SA206356WT_5_luna.rawWild type
Showing results 1 to 32 of 32
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